Phospho-PLC beta 3 (PLCB3)-S1105 Rabbit Polyclonal Antibody for WB and ELISA - Q01970

Core Product Specifications and Parameters

Biological Background: PLC beta 3 (PLCB3) Function and Localization

• PLCB3 encodes phospholipase C beta 3, a key enzyme that hydrolyzes phosphatidylinositol 4,5-bisphosphate to generate second messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (PubMed:20966218, PubMed:29122926, PubMed:37172014). Related references: PMID:20966218, PMID:29122926, PMID:37172014
• It functions as a crucial transducer of G protein-coupled receptor (GPCR) signaling, being directly activated by G(q)/G(11) alpha subunits (PubMed:20966218, PubMed:37991948). Related references: PMID:20966218, PMID:37991948
• In neutrophils, PLCB3 participates in a phospholipase C-activating signaling pathway triggered by N-formyl peptides via GPCRs, leading to RASGRP4 activation by DAG and promoting neutrophil functional responses (By similarity).
• PLCB3, in conjunction with OSBPL2, may regulate keratinocyte proliferation and differentiation, probably through modulation of the ERK pathway and cell cycle (PubMed:38701954). Related references: PMID:38701954
• The protein localizes to the cytoplasm, membrane, and nucleus, reflecting its multifaceted roles in signal transduction.
• Post-translational modifications include phosphorylation and acetylation, and the gene undergoes alternative splicing, producing multiple isoforms.
• Belongs to the phospholipase C family with conserved catalytic domains; the full-length protein has a molecular weight of approximately 139 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to Ser1105; confirm phospho-specificity by comparing signals with non-phosphorylated peptide or phosphatase-treated lysates.
• For Western blot (WB), the predicted molecular weight is ~139 kDa; use appropriate loading controls and consider stripping protocols if probing total PLCB3.
• Validate in relevant species (human, mouse, rat) as indicated; recommend primary cells or tissues with known PLCB3 expression, such as neutrophils or keratinocytes.
• For ELISA, optimize coating and detection conditions; the antibody can be used to quantify phospho-PLCB3 (S1105) levels in cell or tissue extracts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target procurement. This anti-phospho-PLCB3 (S1105) antibody supports research in GPCR signaling and lipid metabolism, critical areas for diagnostic development. From concept to clinic, CamelBio streamlines your supply chain to accelerate assay development.