Phospho-PKR/EIF2AK2-T446 Rabbit mAb - P19525

Core Product Specifications and Parameters

Biological Background: PKR/EIF2AK2 Function and Localization

• Encoded by the EIF2AK2 gene, PKR is an interferon-inducible, double-stranded RNA-activated serine/threonine-protein kinase that also possesses tyrosine kinase activity and belongs to the eIF‑2α kinase family.
• Phosphorylates EIF2S1/eIF‑2‑alpha upon dsRNA binding, triggering the integrated stress response that inhibits global protein synthesis and restricts viral replication. Related references: PMID:18835251, PMID:19189853, PMID:19507191, PMID:21123651, PMID:22948139
• Displays broad antiviral activity against DNA and RNA viruses including HCV, HBV, measles virus, and HSV‑1. Related references: PMID:11836380, PMID:19189853, PMID:19840259
• Modulates apoptosis, proliferation, and differentiation by phosphorylating substrates such as p53, PPP2R5A, DHX9, and IRS1, and regulates signaling pathways (NF‑κB, p38 MAPK, insulin) and transcription factors (STAT1, IRF1, ATF3). Related references: PMID:11836380, PMID:19229320, PMID:22214662, PMID:22948139, PMID:23084476, PMID:23372823, PMID:10848580, PMID:15121867, PMID:15229216, PMID:20685959
• Localizes to the cytoplasm, nucleus, and perinuclear region, with high expression in thymus, spleen, and bone marrow; levels are elevated in Alzheimer’s disease brain and certain carcinomas.
• Key post‑translational modifications include phosphorylation at Thr‑446 (target of this antibody), acetylation, and ubiquitination. Related references: PMID:20395957
• Participates in innate immunity beyond translation control by regulating NLRP3 inflammasome assembly and activation of multiple inflammasomes. Related references: PMID:22801494

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide surrounding Thr‑446 of human PKR; ensure that sample lysis buffers contain phosphatase inhibitors to preserve phosphorylation status.
• This rabbit monoclonal antibody is validated for WB and ELISA; for WB, consider a standard blocking and detection protocol with appropriate loading controls.
• Cross‑reactivity is confirmed for human samples; when working with other species, validate specificity in the relevant sample system.
• Since PKR is activated by dsRNA and interferon, induction conditions may need optimization to detect robust phosphorylation signals.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio offers a comprehensive one‑stop procurement platform for IVD raw materials, supporting researchers and manufacturers working on innate immunity and kinase signaling. With access to validated monoclonal antibodies, optimized phospho‑specific reagents, and bulk ancillary materials, we help streamline your sourcing from target discovery to clinical application. Whether you need rare‑target reagents or large‑volume supplies, CamelBio is ready to assist.