Phospho-PKCalpha/beta II-T638/641 Rabbit pAb for WB, IHC-P, IF/ICC, ELISA - P17252 / P05771

Core Product Specifications and Parameters

Biological Background: PKC alpha/beta II Function and Localization

• Protein kinase C alpha type (PKC alpha) is a calcium-activated, phospholipid- and diacylglycerol-dependent serine/threonine-protein kinase encoded by the PRKCA gene. It plays pivotal roles in regulating cell proliferation, apoptosis, differentiation, migration, adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function, and inflammation.
• PKC alpha localizes to the cytoplasm, cell membrane, mitochondrion membrane, and nucleus, dynamically translocating to specific compartments upon activation to exert its diverse functions.
• During chemokine-induced CD4+ T cell migration, PKC alpha phosphorylates the CDC42-guanine exchange factor DOCK8, leading to its dissociation from LRCH1 and subsequent activation of GTPase CDC42, thereby promoting cell motility. Related references: PMID:28028151
• In response to angiotensin II signaling, PKC alpha phosphorylates KLHL3 at 'Ser-52', decreasing the interaction between KLHL3 and WNK4, which modulates ion transport and blood pressure regulation. Related references: PMID:25313067
• PKC alpha phosphorylates and activates LRRK1, which in turn phosphorylates RAB proteins involved in intracellular trafficking, highlighting a role in vesicular transport. Related references: PMID:36040231
• It is highly expressed in various cancers, including gliomas and breast cancers, where it acts as a tumor promoter and is implicated in malignant phenotypes through regulation of cell proliferation, apoptosis, and migration.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around T638 of human PKCalpha/beta II. Consider validating the antibody in your experimental system by treating cells with PMA or other PKC activators to enhance phosphorylation at T638/641 prior to analysis.
• For Western blotting, use appropriate phosphatase inhibitors during sample preparation to preserve the phospho-epitope. A band around 76–77 kDa is expected.
• In immunohistochemistry (IHC-P), perform antigen retrieval as required and include phosphatase inhibitors in incubation steps to maintain phosphorylation signals.
• For immunofluorescence/immunocytochemistry (IF/ICC), monitor translocation of phosphorylated PKC from cytoplasm to membrane upon activation as a positive control.
• In ELISA, validate the specificity using both phosphorylated and non-phosphorylated peptides to confirm phospho-specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This Phospho-PKCalpha/beta II antibody supports cell signaling and oncology research, and our team is ready to assist with customized sourcing and technical consulting for your projects from concept to clinic.