Phospho-PKC (pan) (βII Ser660) Rabbit Polyclonal Antibody for WB, ELISA - P17252 / P05771 / Q05655 / Q02156 / P24723 / Q04759

Core Product Specifications and Parameters

Biological Background: PKC (pan) (βII Ser660) Function and Localization

• The PKC family comprises multiple serine/threonine kinase isoforms (α, β, δ, ε, η, θ) that regulate key cellular processes; this antibody targets the phosphorylated form of these isoforms at a conserved site corresponding to Ser660 in PKC α (PRKCA).
• PKC α is activated by calcium, phospholipids, and diacylglycerol (DAG), and functions in both positive and negative regulation of cell proliferation, apoptosis, and migration.
• In cancer, PKC α acts as a proto-oncogene, promoting tumor growth in gliomas and breast cancers, and exerts anti-apoptotic effects by phosphorylating BCL2 and suppressing p53-mediated pathways.
• In the immune system, PKC α regulates macrophage differentiation and CD4+ T cell migration; it phosphorylates DOCK8 to activate CDC42 (PubMed:28028151). Related references: PMID:28028151
• PKC α shuttles between the cytoplasm, plasma membrane, mitochondrial membrane, and nucleus, with subcellular localization influencing its substrate access and function.
• In the cardiovascular system, PKC α regulates cardiomyocyte hypertrophy and contractility through pathways involving ERK1/2 and phosphorylation of cardiac troponin T (TNNT2).
• Key post-translational modifications include autophosphorylation, transphosphorylation, and acetylation; phosphorylation at Ser660 is a critical regulatory site for full kinase activity.
• PKC α is also implicated in angiogenesis, platelet aggregation, and inflammation, highlighting its diverse physiological roles.

Experimental Guidance and Technical Tips

• The antibody was raised against a phosphorylated peptide; use phosphatase inhibitors during sample preparation to preserve phosphorylation. Confirm phospho-specificity by treating samples with lambda phosphatase or using non-phosphorylated peptide blocking controls.
• For Western blot, optimize blocking conditions (e.g., BSA instead of milk) to reduce background. Start with an antibody dilution of 1:500–1:2000 and adjust based on preliminary results.
• As a polyclonal antibody, it may recognize multiple PKC isoforms in the predicted molecular weight range; consider isoform-specific siRNA or inhibitors to confirm identity if needed.
• For ELISA, ensure compatibility of coating antigens and validate with known positive and negative controls.
• With cross-reactivity to mouse and rat, this antibody is suitable for rodent models; verify reactivity in your specific tissue or cell line with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This pan-PKC phospho-specific antibody resource supports cutting-edge research in signal transduction, oncology, and cardiovascular biology, enabling streamlined procurement and development workflows.