Phospho-PKC mu (S916) Rabbit Polyclonal Antibody for WB and ELISA - Q15139

Core Product Specifications and Parameters

Biological Background: Serine/threonine-protein Kinase D1 (PRKD1) Function and Localization

• Serine/threonine-protein kinase D1 (PRKD1), also known as PKC mu or nPKC-D1, is a member of the protein kinase D family that responds to diacylglycerol signals.
• Converts transient diacylglycerol (DAG) signals into sustained physiological responses by regulating MAPK8/JNK1, Ras signaling, Golgi membrane integrity, and NF-kappa-B-mediated cell survival. Related references: PMID:10764790 PMID:12505989 PMID:12637538
• Is critical for cell migration, osteoblast differentiation via HDAC7 nuclear export, and neuronal polarity maintenance. Related references: PMID:18509061
• Mediates cardiac hypertrophy through HDAC5 phosphorylation and pathological remodeling, and promotes VEGFA-induced angiogenesis via HDAC7 phosphorylation. Related references: PMID:18332134 PMID:19211839
• Under genotoxic stress, is cleaved by caspase-3 to activate kinase function and enhance apoptosis; also involved in flagellin-stimulated inflammatory responses by phosphorylating TLR5. Related references: PMID:10764790 PMID:17442957
• Phosphorylates EGFR on threonine residues, leading to suppression of EGF-induced MAPK8/JNK1 activation. Related references: PMID:10523301
• Localizes to cytoplasm, cell membrane, and trans-Golgi network; under oxidative stress, is phosphorylated at Tyr-463 via SRC-ABL1 to promote cell survival. Related references: PMID:12505989

Experimental Guidance and Technical Tips

• Use phosphatase inhibitors during sample preparation to preserve the phosphorylation at Ser916.
• Validate the antibody in your cell line or tissue of interest; consider using a pan-PRKD1 antibody for normalization.
• For Western blot, a band around 102 kDa is expected; optimize blocking and antibody dilutions.
• For ELISA, the antibody may be used as a capture or detection reagent; titrate for optimal performance.
• Note that this antibody was raised against a peptide corresponding to human PRKD1 Ser912 (homologous to Ser916), so cross-reactivity with other phospho-sites or species should be verified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop platform for IVD raw material sourcing, offering validated antibodies, matched pairs, and bulk reagents. This anti-phospho-PRKD1 (PKC mu) antibody complements our kinase signaling portfolio, supporting assay development for cardiovascular, oncology, and inflammation research. Our team assists with rare target raw-material sourcing and technical consulting to accelerate your diagnostic project from concept to clinic.