Phospho-PKC alpha-T638 + PKC beta II-T641 Rabbit Monoclonal Antibody for WB, ELISA - P17252/P05771

Core Product Specifications and Parameters

Biological Background: PKC alpha and PKC beta Function and Localization

• This antibody recognizes the phosphorylated forms of protein kinase C alpha (PRKCA) at Thr638 and protein kinase C beta II (PRKCB) at Thr641, both members of the serine/threonine kinase PKC family.
• PKC alpha is a calcium-, phospholipid- and diacylglycerol-dependent kinase involved in diverse cellular processes such as proliferation, apoptosis, differentiation, migration, adhesion, tumorigenesis, cardiac hypertrophy, and angiogenesis.
• It dynamically localizes to the cytoplasm, cell membrane, mitochondrial membrane, and nucleus, depending on cellular context and stimuli.
• In chemokine-induced CD4+ T cell migration, phosphorylates DOCK8, leading to CDC42 activation. Related references: PMID:28028151
• In response to angiotensin II, phosphorylates KLHL3, reducing its interaction with WNK4 and modulating ion homeostasis. Related references: PMID:25313067
• Phosphorylates and activates LRRK1, which in turn phosphorylates RAB proteins to regulate intracellular trafficking. Related references: PMID:36040231
• PKC alpha is highly expressed in numerous cancers and can act as a tumor promoter, with implications in gliomas and breast cancers.
• It plays critical roles in angiogenesis and platelet aggregation through VEGFA-dependent signaling and CD36/RAP1GAP-mediated pathways.

Experimental Guidance and Technical Tips

• The antibody is raised against a phosphorylated peptide; consider treating samples with lambda phosphatase to confirm phosphorylation-specific binding.
• For western blot, bands are expected near 76 kDa (PKC alpha) and 77 kDa (PKC beta II). Optimize gel percentage and transfer conditions for proteins in this range.
• Validate specificity by including both phosphorylated and non-phosphorylated peptide controls in ELISA.
• Choose relevant cell lines or tissue models known to express active PKC alpha and PKC beta II, and consider appropriate stimuli (e.g., phorbol ester) to induce phosphorylation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic developers and researchers with reliable access to validated antibodies and ancillary reagents. This phospho-specific PKC antibody, targeting key signaling nodes in cancer, cardiovascular, and inflammatory pathways, aligns with our capability to supply optimized monoclonal/polyclonal raw materials for assay development. Beyond individual antibodies, we facilitate procurement of bulk reagents, matched pairs, and rare-target materials to streamline your project from concept to clinical application.