Phospho-PKC alpha-T410+PKC delta-T410 Rabbit mAb for WB, ELISA - P17252 / Q05655

Core Product Specifications and Parameters

Biological Background: PKC alpha and PKC delta Function and Localization

• PRKCA (PKC alpha) is a calcium-activated, phospholipid- and DAG-dependent serine/threonine-protein kinase of the PKC family, which also includes PRKCD (PKC delta). It regulates cell proliferation, apoptosis, differentiation, migration, and adhesion, and is highly expressed in various cancers, acting as a tumor promoter.
• PRKCA phosphorylates key substrates such as RAF1, BCL2, and TNNT2, thereby modulating the MAPK/ERK signaling cascade and apoptosis. For instance, phosphorylation of BCL2 promotes cell survival in leukemia cells.
• PRKCA plays a critical role in cell motility: upon wounding, it translocates to lamellipodia and phosphorylates CSPG4, inducing cytoskeletal rearrangement and polarized migration.
• The kinase localizes to the cytoplasm, cell membrane, mitochondrial membrane, and nucleus, with stimulus-induced translocation being vital for its context-specific functions.
• Phosphorylation of the activation loop (T410 in PKC delta, equivalent in PKC alpha) is essential for kinase activity. This monoclonal antibody specifically recognizes PKC alpha and delta phosphorylated at T410.
• In the cardiovascular system, PRKCA negatively regulates myocardial contractility and promotes angiogenesis and cardiomyocyte hypertrophy, in part through MAPK1/3 signaling, linking it to heart failure.
• PRKCA phosphorylates DOCK8 to control CDC42 in T cell migration (PubMed:28028151) and phosphorylates KLHL3 in renal ion transport (PubMed:25313067). It also activates LRRK1 to regulate RAB protein trafficking (PubMed:36040231). Related references:
  • PMID:28028151
  • PMID:25313067
  • PMID:36040231

Experimental Guidance and Technical Tips

• The immunogen was a synthetic phosphopeptide corresponding to residues around T410 of human PRKCZ, which shares homology with the activation loop sequences of PKC alpha and delta. Users should verify that the antibody recognizes the expected phospho-proteins in their system.
• Recommended applications include WB and ELISA. For WB, the predicted molecular weights are 76 kDa (PKC alpha) and 77 kDa (PKC delta). Use appropriate positive (e.g., PMA-stimulated cells) and negative (phosphatase-treated or unstimulated) controls.
• To confirm phospho-specificity, consider performing a phosphatase treatment of lysates before WB analysis.
• For ELISA, follow standard phospho-antibody protocols and optimize coating and detection conditions for sensitivity.
• Since the antibody is a rabbit monoclonal, it offers high reproducibility and lot-to-lot consistency, suitable for quantitative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a comprehensive one-stop sourcing solution for IVD raw materials. This anti-phospho-PKC alpha/delta antibody supports research into cancer signaling, cardiovascular disease, and inflammation. Beyond this product, we offer validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents, ensuring seamless scale-up from discovery to clinical applications.