Phospho-PKC alpha-S657/Y658 Rabbit Polyclonal Antibody for WB, ELISA - P17252

Core Product Specifications and Parameters

Biological Background: PKC alpha Function and Localization

• Protein kinase C alpha type (PKC alpha) is a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that regulates cell proliferation, apoptosis, differentiation, migration, adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function, and inflammation.
• Plays dual roles in cell growth: promotes proliferation via RAF1/MAPK/ERK signaling and can induce cell cycle arrest through hyperphosphorylation of RB1 and upregulation of CDK inhibitors CDKN1A and CDKN1B in certain contexts.
• Exhibits anti-apoptotic functions in cancer cells by suppressing p53/TP53-mediated IGFBP3 activation and phosphorylating BCL2 in leukemia, contributing to tumor promoter activity in gliomas and breast cancers.
• In CD4+ T cell chemokine-induced migration, phosphorylates DOCK8, causing its dissociation from LRCH1 and activation of CDC42 GTPase. Related references: PMID:28028151
• In the kidney, phosphorylates KLHL3 in response to angiotensin II signaling, disrupting the KLHL3-WNK4 interaction and modulating ion homeostasis. Related references: PMID:25313067
• Phosphorylates and activates LRRK1, which in turn phosphorylates RAB proteins involved in intracellular trafficking. Related references: PMID:36040231
• Subcellular localization includes cytoplasm, cell membrane, mitochondrion membrane, and nucleus, enabling signal transduction at multiple sites.
• Classified as a proto-oncogene and phosphoprotein with zinc-finger domains, belonging to the AGC kinase family.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide encompassing residues around S657 and Y658; consider verifying phospho-specificity by treating samples with phosphatase or using non-phosphorylated peptide competition.
• For Western blotting, the predicted molecular weight is 77 kDa; validate band specificity in the relevant cell or tissue lysate system, possibly after stimulation with known PKC activators (e.g., PMA).
• In ELISA applications, optimize coating antigen and antibody dilutions; cross-reactivity is reported for human, but testing on other species may be needed if conservation of the phosphorylation site is high.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a single access point for IVD raw materials, technical services, and consulting from concept to clinic. This phospho-specific PKC alpha antibody supports research into cancer signaling, cardiovascular biology, and inflammation pathways. Our sourcing capabilities extend to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and procurement of rare-target raw materials for your assay development needs.