Phospho-PKA C-alpha (PRKACA)-T197 Rabbit pAb - P17612

Core Product Specifications and Parameters

Biological Background: PKA C-alpha Function and Localization

• cAMP-dependent protein kinase catalytic subunit alpha (PRKACA) is a serine/threonine kinase that phosphorylates numerous substrates in response to cAMP, regulating key cellular processes including metabolism, transcription, and cell cycle (PubMed:15642694, PubMed:15905176, PubMed:16387847). Related references: PMID:15642694 PMID:15905176 PMID:16387847
• PRKACA localizes to multiple subcellular compartments, including cytoplasm, nucleus, cell membrane, mitochondrion, and cilia, enabling spatially restricted signaling.
• Two isoforms exist: isoform 1 is ubiquitously expressed, while isoform 2 is sperm-specific and enriched in pachytene spermatocytes, reflecting distinct tissue functions.
• The kinase is regulated by cAMP-mediated dissociation from regulatory subunits and by phosphorylation; it is a phosphoprotein with multiple modification sites.
• PRKACA acts as a key inhibitor of the smoothened signaling pathway by phosphorylating GLI transcription factors, preventing their transcriptional activation in the absence of hedgehog ligands (PubMed:16705181). Related references: PMID:16705181
• Mutations in PRKACA are associated with Cushing syndrome, illustrating its critical role in endocrine regulation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide spanning the T197 region; as a phospho-specific antibody, consider validating specificity using phosphatase-treated lysates alongside positive controls.
• For WB applications, anticipate a single band at approximately 41 kDa; include phosphatase inhibitors in lysis buffers to preserve phosphorylation.
• Cross-reactivity with mouse and rat is reported; sequence alignment of the T197 motif across species is advisable to confirm epitope conservation.
• ELISA experiments may benefit from coating with the phospho-peptide and using non-phosphorylated peptide as a negative control to ensure phospho-selectivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive sourcing for IVD raw materials, including phospho-specific antibodies like this anti-PKA C-alpha (p-T197) rabbit pAb, alongside complementary reagents for kinase signaling research. As your one-stop partner, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline assay development from concept to clinic.