Phospho-PI3KP85α/P55γ/P85β-Y467/Y464/Y199 Rabbit Polyclonal Antibody for WB - P27986 / O00459 / Q92569

Core Product Specifications and Parameters

Biological Background: PI3K Regulatory Subunits (p85α/p55γ/p85β) Function and Localization

• PIK3R1 encodes the phosphatidylinositol 3-kinase regulatory subunit alpha, also known as PI3-kinase p85-alpha. Multiple isoforms exist via alternative splicing, including isoform 2 which is expressed in skeletal muscle and brain.
• The p85-alpha subunit acts as an adapter, binding to activated (phosphorylated) protein-tyrosine kinases via its SH2 domain, mediating the association of the catalytic p110 subunit to the plasma membrane.
• It is necessary for insulin-stimulated glucose uptake and glycogen synthesis in insulin-sensitive tissues, playing a key role in insulin signaling.
• Plays a role in signaling downstream of various growth factor receptors including FGFR1-4, KIT, PDGFRA, and PDGFRB, as well as integrin ITGB2 signaling. Related references: PMID:17626883 PMID:19805105 PMID:7518429
• Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2, contributing to glucose tolerance improvement under metabolic overloading in the liver. Related references: PMID:20348923
• Localizes primarily to the cytoplasm, where it interacts with multiple signaling partners.
• Post-translational modifications include phosphorylation (on tyrosine residues such as Y467), acetylation, and ubiquitination, regulating its activity and stability.
• Dysregulation of PI3K pathway components is implicated in cancer, metabolic disorders, and developmental diseases like dwarfism.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho-specific peptide corresponding to residues surrounding Y467 of human PIK3R1/PIK3R2/PIK3R3. Due to sequence conservation, the antibody cross-reacts with mouse and rat species.
• For Western blot, denature samples in Laemmli buffer and load 20–30 µg of total protein per lane. The detected bands may appear between 42–54 kDa (p55γ/p85β) and 81–84 kDa (p85α), depending on the specific isoform.
• Phosphorylation-specific antibodies require careful handling: avoid repeated freeze-thaw cycles and consider using phosphatase inhibitors during sample preparation to preserve phosphorylation signals.
• Include appropriate positive controls (e.g., insulin-stimulated or growth factor-treated cell lysates) and negative controls (non-phosphorylated peptide blocking or phosphatase treatment) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material supplier, CamelBio offers comprehensive access to high-quality antibodies, including phosphorylated and isoform-specific reagents like this anti-PI3K regulatory subunit antibody. We cater to diagnostic manufacturers, CROs, and research institutes with a focus on streamlined procurement and technical support. Our portfolio spans validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents essential for assay development in the PI3K/Akt signaling axis—a key pathway in oncology and metabolic disease research. Reach out to explore custom sourcing solutions for your next project.