Phospho-PFKFB2-S483 Rabbit pAb for WB, ELISA - O60825

Core Product Specifications and Parameters

Biological Background: PFKFB2 Function and Localization

• PFKFB2 encodes 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2, also known as the heart-type isozyme (6PF-2-K/Fru-2,6-P2ase), a bifunctional enzyme regulating fructose-2,6-bisphosphate levels.
• The enzyme catalyzes both the synthesis and degradation of fructose 2,6-bisphosphate, a key allosteric activator of glycolysis, thereby controlling glycolytic flux in the heart.
• PFKFB2 is predominantly expressed in cardiac tissue, highlighting its critical role in cardiac energy metabolism and contractile function.
• The protein belongs to the phosphofructokinase-2 family and carries multiple post-translational modifications, including phosphorylation, acetylation, and alternative splicing, modulating its activity and subcellular distribution.
• Phosphorylation at Ser483, the epitope recognized by this antibody, represents a specific activation signal that integrates hormonal and metabolic cues.

Experimental Guidance and Technical Tips

• When performing Western blot, consider verifying the specificity of the phospho-specific signal by comparing with a non-phosphorylated peptide blocking control or by treating samples with phosphatase.
• For ELISA applications, ensure the coating antigen or capture antibody is compatible with phospho-peptide recognition, and include a non-phosphorylated peptide control to assess binding specificity.
• Since this antibody is raised against a synthetic peptide corresponding to the phosphorylated S483 epitope, it is advisable to validate its reactivity in the relevant sample system (e.g., human heart tissue lysates or PFKFB2-overexpressing cells) and optimize working dilutions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for diagnostic manufacturers and research laboratories, offering validated antibody pairs, optimized recombinant proteins, and ancillary reagents. This phospho-PFKFB2-S483 antibody, targeting cardiac fructose-2,6-bisphosphate metabolism, complements our portfolio of signal transduction and metabolic disorder research tools. For bulk orders or custom sourcing of rare targets, contact CamelBio to accelerate your concept-to-clinic development.