Phospho-PEA15-S104 Rabbit Polyclonal Antibody for WB, ELISA - Q15121

Core Product Specifications and Parameters

Biological Background: Astrocytic Phosphoprotein PEA-15 Function and Localization

• PEA-15 (Phosphoprotein Enriched in Astrocytes) is a 15 kDa cytoplasmic protein encoded by the PEA15 gene. Alternative splicing produces multiple isoforms.
• It acts as a multifunctional adaptor, blocking Ras-mediated inhibition of integrin activation and modulating the ERK MAP kinase cascade.
• PEA-15 inhibits apoptosis by suppressing both TNFRSF6- and TNFRSF1A-mediated CASP8 activation.
• It retains RPS6KA3 in the cytoplasm, thereby inhibiting its activities (By similarity).
• The protein plays a key role in glucose transport by regulating the plasma membrane levels of SLC2A1 and the insulin-dependent trafficking of SLC2A4.
• Ubiquitously expressed, with highest levels in heart, brain, muscle, and adipose tissue; expression is elevated in tissues from individuals with type 2 diabetes.
• The S104 phosphorylation site is critical for modulating PEA-15 function.
• Keywords: Apoptosis, Sugar transport, Phosphoprotein, Alternative splicing, Cytoplasm.

Experimental Guidance and Technical Tips

• This antibody was generated against a synthetic phosphopeptide corresponding to residues surrounding S104 of human PEA-15; it is expected to specifically recognize the phosphorylated form.
• For Western blot, a band around 15 kDa is anticipated; confirm band specificity by including phosphatase-treated controls.
• When performing ELISA, coat plates with the phosphopeptide antigen or cell lysates; validate binding specificity under non-denaturing conditions.
• Cross-reactivity with mouse and rat is predicted based on sequence conservation; verify in the relevant sample system before extended studies.
• Include phosphatase inhibitors during sample preparation to preserve phosphorylation signals.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-phospho-PEA15-S104 rabbit polyclonal antibody supports studies on apoptosis, glucose transport, and diabetes-related signaling pathways. For custom phospho-specific antibody sourcing or rare target raw materials, CamelBio’s supply chain streamlines procurement from concept to clinic.