Phospho-PDK1/PDPK1-S241 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O15530

Core Product Specifications and Parameters

Biological Background: PDK1/PDPK1 Function and Localization

• PDK1 (PDPK1) is a master serine/threonine kinase that phosphorylates and activates a subgroup of AGC family kinases, including AKT/PKB, p70 S6 kinase, p90 RSK, PKC, SGK, and PAK1, thereby regulating cell proliferation, survival, metabolism, and gene expression. Related references: PMID:10226025, PMID:10480933, PMID:10995762
• It plays a central role in insulin signal transduction by phosphorylating AKT/PKB, which propagates downstream signals controlling glucose uptake, amino acid uptake, storage, cell proliferation, and survival. Related references: PMID:10226025, PMID:12167717, PMID:9094314
• PDK1 negatively regulates TGF-beta signaling by modulating SMAD protein interactions and phosphorylation, and by preventing nuclear translocation of SMAD3 and SMAD4. Related references: PMID:17327236
• It activates the NF-kappa-B pathway via phosphorylation of IKKB, contributing to inflammatory responses. Related references: PMID:16207722
• The tyrosine-phosphorylated form of PDK1 is crucial for focal adhesion dynamics in response to angiotensin II. Related references: PMID:14585963
• PDK1 is essential for vascular endothelial cell motility and chemotaxis, playing a role in angiogenesis. Related references: PMID:17371830
• It localizes to the cytoplasm, nucleus, cell membrane, and focal adhesions, reflecting its multifunctional roles.
• PDK1 is ubiquitously expressed; the Tyr-9 phosphorylated form is elevated in diseased tissues from lung, liver, colon, and breast.
• Alternative splicing produces a catalytically inactive isoform; the protein undergoes acetylation and ubiquitin-like conjugation.

Experimental Guidance and Technical Tips

• This antibody was raised against a phospho-specific peptide corresponding to residues surrounding Ser241 of human PDK1; it detects the phosphorylated form. For WB and IF/ICC, use phosphatase inhibitors in lysis and fixation buffers.
• For WB, a band at ~63 kDa is expected. Use stimulated vs. unstimulated cell lysates (e.g., insulin-treated) as positive/negative controls.
• Cross-reactivity with mouse and rat allows for studies in these species; validate antibody specificity with appropriate controls.
• For ELISA, consider using a phosphorylated peptide as standard and a non-phosphorylated peptide to confirm phospho-specificity.
• Optimize antibody dilution for each application; suggested starting ranges can be determined experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop sourcing for IVD raw materials, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This PDK1 phospho-specific antibody supports research in kinase signaling, insulin pathway, and cancer biology. Contact us for customized procurement and technical consultation tailored to your diagnostic development needs.