Phospho-PDK1/PDPK1-S241 Rabbit pAb - O15530

Core Product Specifications and Parameters

Biological Background: PDK1 (3-phosphoinositide-dependent protein kinase 1) Function and Localization

• PDPK1 (PDK1) encodes a ubiquitously expressed serine/threonine kinase that serves as a master regulator of the AGC kinase family, including PKB/AKT, p70 S6K, p90 RSK, and PKC isoforms. Related references: PMID:10226025 PMID:10480933 PMID:10995762

• In insulin signaling, PDK1 phosphorylates and activates AKT1 at Thr308, propagating downstream signals that control glucose uptake, glycogen synthesis, and cell survival. Related references: PMID:10226025 PMID:12167717 PMID:9094314

• PDK1 localizes to the cytoplasm, nucleus, cell membrane, and focal adhesions, enabling diverse signaling from multiple cellular compartments.

• The tyrosine-phosphorylated form of PDK1 is essential for focal adhesion dynamics and is upregulated in diseased tissues of the lung, liver, colon, and breast. Related references: PMID:14585963

• PDK1 modulates additional pathways: it negatively regulates TGF-beta signaling by phosphorylating SMAD proteins and activates NF-κB via IKKB phosphorylation, influencing inflammatory and immune responses. Related references: PMID:17327236 PMID:16207722

• PDK1 is critical for vascular endothelial cell motility and chemotaxis, participating in angiogenic processes. Related references: PMID:17371830

Experimental Guidance and Technical Tips

• Phospho-specific antibodies require careful sample handling: add phosphatase inhibitors to lysis buffers and avoid prolonged sample incubation at room temperature.
• For Western blotting, treat a portion of the sample with lambda phosphatase to confirm the phospho-specificity of the band at ~63 kDa.
• In immunofluorescence (IF/ICC), fixation and permeabilization conditions may affect epitope accessibility; test multiple fixation methods (e.g., methanol vs. formaldehyde) for optimal signal.
• Given cross-reactivity with mouse and rat, validate the antibody in the intended species model by sequence alignment of the phosphorylation site and antigen region.
• ELISA applications may benefit from using a phospho-peptide competitor to ensure signal specificity.

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CamelBio supports diagnostic manufacturers with a comprehensive portfolio of IVD raw materials, including high-quality phospho-specific antibodies like this anti-phospho-PDK1-S241 pAb. Our sourcing network provides matched antibody pairs, bulk ancillary reagents, and custom production capabilities to accelerate your assay development from concept to clinic. Rely on CamelBio for reliable access to validated critical raw materials for signal transduction and oncology biomarker detection.