Phospho-PDHA1-S293 Rabbit mAb - P08559

Core Product Specifications and Parameters

Biological Background: PDHA1 Function and Localization

• PDHA1 (Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial), also known as PDHE1-A type I, is encoded by the PDHA1 gene in humans. It is a crucial metabolic enzyme that, together with PDHB, constitutes the heterotetrameric E1 subunit of the pyruvate dehydrogenase (PDH) complex.
• The PDH complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, linking cytoplasmic glycolysis to the mitochondrial tricarboxylic acid (TCA) cycle. Related references: PMID:19081061 PMID:7782287
• Specifically, the E1 subunit catalyzes thiamine pyrophosphate (TPP)-dependent decarboxylation of pyruvate and the reductive acetylation of lipoyl groups covalently attached to the E2 component. Related references: PMID:17474719 PMID:19081061 PMID:7782287
• PDHA1 belongs to the pyruvate dehydrogenase complex gene family; mutations in this gene are associated with primary mitochondrial disease and Leigh syndrome.
• Subcellularly, PDHA1 localizes to the mitochondrion matrix, consistent with its role in pyruvate metabolism.
• Post-translational modifications include phosphorylation (e.g., at Ser293) and acetylation, which modulate enzyme activity. Keywords: Acetylation, Phosphoprotein, Thiamine pyrophosphate, Magnesium, Metal-binding.
• The protein is ubiquitously expressed across tissues.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide corresponding to residues surrounding Ser293 of human PDHA1, targeting a phosphorylation-specific epitope. This makes the antibody suitable for detecting endogenously phosphorylated PDHA1 under appropriate stimulation conditions.
• For Western blotting, PDHA1 has a predicted molecular weight of 43 kDa; consider optimizing blocking and primary antibody dilution using lysates from human, mouse, or rat cells/tissues.
• For immunoprecipitation, the rabbit monoclonal format may offer high specificity; validate capture efficiency with phospho-positive and -negative controls.
• ELISA applications are validated; optimize coating antigen and detection conditions for quantitative phospho-analysis.
• Reported cross-reactivity includes mouse and rat; verify sequence homology around the phosphorylation site if extending to other species.

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CamelBio supports diagnostic manufacturers, laboratories, and research institutes with comprehensive access to high-quality IVD raw materials, including validated phospho-specific antibodies like this PDHA1-S293 rabbit mAb for mitochondrial metabolism research. Our supply capabilities encompass antibody pairs, bulk ancillary reagents, and rare-target sourcing to accelerate your assay development from concept to clinic.