Phospho-PDGFRB-Y740 Rabbit pAb for WB and ELISA - P09619

Core Product Specifications and Parameters

Biological Background: PDGFRB Function and Localization

• Platelet-derived growth factor receptor beta (PDGFRB) is a receptor tyrosine kinase that binds homodimeric PDGFB and PDGFD, as well as PDGFA/PDGFB heterodimers, triggering intracellular signaling cascades.
• PDGFRB plays essential roles in embryonic development, promoting cell proliferation, survival, differentiation, chemotaxis, and migration.
• It is critical for blood vessel development by driving proliferation, migration, and recruitment of pericytes and smooth muscle cells to endothelial cells, and for normal formation of the kidney glomerular capillary network.
• Upon ligand binding, PDGFRB autophosphorylates at multiple tyrosine residues (including Y740), creating docking sites for SH2 domain-containing proteins such as PI3K, PLCγ1, and GRB2, leading to activation of the AKT, PKC, and MAPK/ERK pathways.
• PDGFRB localizes primarily to the cell membrane, and upon activation is internalized into cytoplasmic vesicles and lysosomes for signal termination and receptor degradation.
• The receptor is subject to alternative splicing and chromosomal rearrangements, some of which are implicated in certain leukemias and solid tumors; it is also known as CD140B, PDGFR1, and PDGFR-1.
• PDGFRB is classified as a phosphoprotein, glycoprotein, and proto-oncogene, underscoring its importance in signal transduction and cancer biology.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Tyr740; for Western blot, consider using PDGF-stimulated cell lysates as a positive control and unstimulated or phosphatase-treated lysates to verify phospho-specificity.
• For ELISA, a phosphorylated peptide standard is recommended to establish quantification; non-phosphorylated peptide can be used to assess antibody specificity.
• Optimal working dilutions and conditions should be determined empirically; validate the antibody in the relevant species and sample system.
• Cross-reactivity with human and mouse is indicated, but performance in other species or applications should be tested by the end user.

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