Phospho-PDGFR alpha-Y849 Rabbit pAb for WB, ELISA - P16234

Core Product Specifications and Parameters

Biological Background: PDGFRA Function and Localization

• Platelet-derived growth factor receptor alpha (PDGFRA, also known as Alpha platelet-derived growth factor receptor, CD140a antigen, PDGFR-2) is a tyrosine-protein kinase receptor that binds PDGFA, PDGFB, and PDGFC ligands, initiating intracellular signaling cascades.
• PDGFRA plays a critical role in embryonic development, regulating processes such as cell proliferation, survival, chemotaxis, and differentiation of bone marrow-derived mesenchymal stem cells; it is essential for normal skeleton development and cephalic closure.
• Upon ligand binding, PDGFRA activates multiple pathways including PI3K-AKT1, PLCG1-mediated calcium mobilization and PKC, HRAS-MAPK/ERK, and STAT1/3/5, with signaling modulated by heterodimer formation with PDGFRB.
• In wound healing, PDGFRA promotes cell migration and chemotaxis; it also contributes to platelet activation, agonist secretion, and thrombin-induced platelet aggregation.
• Subcellular localization includes the cell membrane, primary cilium, and Golgi apparatus; the receptor undergoes internalization and down-regulation by protein phosphatases after activation.
• Detected at the protein level in platelets and widely expressed in tissues such as brain, fibroblasts, smooth muscle, heart, and embryo; it is found in both normal and tumor colon tissues.
• Keyword-annotated domains and features include immunoglobulin-like domains, transmembrane helix, ATP-binding site, tyrosine kinase activity, disulfide bonds, N-glycosylation, phosphorylation sites, and ubiquitination.

Experimental Guidance and Technical Tips

• This phospho-specific antibody was raised against a synthetic peptide encompassing phosphorylated Y849 of human PDGFRA; it is expected to recognize the phosphorylated form and may not detect the non-phosphorylated receptor.
• For Western blotting, prepare cell or tissue lysates using buffers supplemented with phosphatase inhibitors to preserve phospho-epitopes; the predicted molecular weight is approximately 123 kDa.
• In ELISA, consider pairing the antibody with an appropriate capture or detection system; optimal antibody titration and blocking conditions should be determined empirically.
• As cross-reactivity is listed for human only, verify specificity in the relevant human sample system; PDGFRA is endogenously expressed in many cell types and can be induced by PDGF stimulation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this phospho-specific PDGFRA antibody alongside a comprehensive portfolio of IVD raw materials, including validated antibody pairs and bulk ancillary reagents. Our one-stop sourcing services support diagnostic manufacturers and researchers in obtaining reliable immunoassay components, with expert technical guidance from concept to clinic. For studies on PDGF signaling and its roles in development and disease, we provide flexible supply solutions—please consult us for your specific needs.