Phospho-Paxillin (Y118) Rabbit Polyclonal Antibody for WB and ELISA - P49023

Core Product Specifications and Parameters

Biological Background: Paxillin Function and Localization

• Paxillin is a multidomain scaffold protein that localizes to focal adhesions, serving as a crucial adaptor for integrin-mediated cell adhesion and signaling.
• It contains multiple protein-interaction motifs, including LIM domains and LD motifs, which facilitate binding to structural and signaling proteins such as vinculin, FAK, and Src.
• Paxillin plays a central role in actin cytoskeleton organization by linking the actin-membrane interface at focal adhesions, thereby regulating cell morphology and motility.
• Through its phosphorylation at specific tyrosine residues (e.g., Y31, Y118, Y181), paxillin acts as a docking site for SH2 domain-containing proteins, modulating downstream signaling cascades.
• The protein is involved in recruiting other regulatory molecules to adhesion sites, as evidenced by its interaction with TRIM15 at focal adhesions.
• Subcellularly, paxillin is predominantly found at focal adhesions, but also associates with the cell cortex and cytoplasmic compartments.
• It is subject to multiple post-translational modifications, including acetylation and phosphorylation, influencing its stability and protein interactions.
• The LIM domains in paxillin are zinc-binding motifs, contributing to protein-protein interactions and targeting to adhesion complexes.

Experimental Guidance and Technical Tips

• This antibody was generated using a synthetic phosphorylated peptide corresponding to tyrosine 118 of human paxillin, making it suitable for detecting phosphorylation levels at this site.
• For Western blotting, a band of approximately 65 kDa is expected; validate specificity using phosphatase treatment or site-mutated controls.
• In ELISA applications, consider using the phosphopeptide as a positive control and the corresponding non-phosphorylated peptide to assess phosphorylation-specific binding.
• Cross-reactivity is reported for human; verify reactivity with other species if needed.
• The antibody is supplied as a rabbit polyclonal, so lot-to-lot consistency may vary; consider titrating the antibody for optimal performance in your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to IVD raw materials, including phospho-specific antibodies like this anti-phospho-paxillin (Y118) polyclonal antibody. Our portfolio spans validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, supporting every stage from assay development to commercialization. Let CamelBio be your reliable sourcing partner for cell adhesion and signaling research raw materials.