Phospho-PAK2-S20 Rabbit Polyclonal Antibody for WB & ELISA - Q13177

Core Product Specifications and Parameters

Biological Background: PAK2 Function and Localization

• PAK2 is a serine/threonine kinase that functions as a downstream effector of CDC42 and RAC1, undergoing autophosphorylation upon GTPase binding to regulate cytoskeleton dynamics, cell motility, and proliferation (PubMed:7744004, PubMed:12853446, PubMed:16617111, PubMed:19273597).
  Related references:
  PMID:7744004 PMID:12853446 PMID:16617111 PMID:19273597
• Full-length PAK2 promotes cell survival, while caspase cleavage at DNA damage yields a pro-apoptotic p34 fragment that translocates to the nucleus and activates the JNK pathway (PubMed:12853446, PubMed:16617111, PubMed:9171063).
  Related references:
  PMID:12853446 PMID:16617111 PMID:9171063
• Its kinase activity phosphorylates numerous substrates: histone H4 for nucleosome assembly, JUN for EGF-induced proliferation, MAPK4/6 to regulate migration, and CASP7 to prevent apoptosis (PubMed:21724829, PubMed:21177766, PubMed:21317288, PubMed:21555521, PubMed:27889207).
  Related references:
  PMID:21724829 PMID:21177766 PMID:21317288 PMID:21555521 PMID:27889207
• Kinase-independent roles involve mitotic spindle orientation through association with ARHGEF7 and GIT1 (PubMed:19273597, PubMed:19923322).
  Related references:
  PMID:19273597 PMID:19923322
• Ubiquitously expressed with highest levels in skeletal muscle, ovary, thymus, and spleen.
• Shows dynamic subcellular distribution, localizing to cytoplasm, nucleus, perinuclear region, and membrane.

Experimental Guidance and Technical Tips

• For Western blot, include phosphatase inhibitors in lysis buffer to preserve phosphorylation. Verify specificity by treating samples with lambda phosphatase.
• The immunogen is a phosphopeptide around Ser20; cross-reactivity with unphosphorylated PAK2 may be minimal but should be validated.
• Suitable for detecting PAK2 activation in cell lines treated with growth factors or apoptotic stimuli; consider including positive and negative controls.
• Antibody has been reported to work in ELISA; appropriate coating and blocking conditions should be optimized.
• Cross-reactivity with mouse and rat PAK2 is indicated; sequence alignment shows high conservation around the phosphorylation site, but local validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic manufacturers and research laboratories by offering a comprehensive portfolio of IVD raw materials. This phospho-PAK2 (S20) antibody supports investigations into PAK2-mediated signaling and apoptosis. Our sourcing solutions provide access to validated antibodies, recombinant proteins, and ancillary reagents to accelerate assay development.