Phospho-p90Rsk/RSK1/RPS6KA1-T359/S363 Rabbit Polyclonal Antibody for WB, ELISA - Q15418

Core Product Specifications and Parameters

Biological Background: RPS6KA1/RSK1 Function and Localization

• Protein identity: RPS6KA1 encodes the 90 kDa ribosomal protein S6 kinase alpha-1 (RSK1/p90RSK), a serine/threonine kinase of the p90RSK family; alternative splicing generates multiple isoforms.
• ERK-MAPK pathway effector: Operates downstream of ERK1/2 to phosphorylate transcription factors CREB1, ETV1/ER81, NR4A1/NUR77, and the cofactor CREBBP, promoting immediate-early gene expression and mitogenesis.
  Related references: PMID:18508509 PMID:18813292 PMID:12213813 PMID:16223362
• Translation initiation: Enhances cap-dependent translation by phosphorylating RPS6 and EIF4B in response to insulin or growth factors.
  Related references: PMID:16763566 PMID:17360704
• mTOR signaling modulator: Regulates mTORC1 and mTORC2 activity through phosphorylation of TSC2 and RPTOR, and feeds back via DEPTOR.
  Related references: PMID:15342917 PMID:22017876
• Anti-apoptotic effects: Inhibits pro-apoptotic proteins BAD and DAPK1 and promotes survival of hepatic stellate cells and hepatocytes via CEBPB phosphorylation.
  Related references: PMID:10679322 PMID:16213824 PMID:11684016 PMID:18508509 PMID:18813292
• Cell migration: Phosphorylates EPHA2 at Ser-897 to control cell motility.
  Related references: PMID:26158630
• Subcellular localization and regulation: Shuttles between nucleus and cytoplasm; kinase activity is controlled by multi-site phosphorylation, including the activation-relevant Thr359 and Ser363 residues recognized by this antibody.

Experimental Guidance and Technical Tips

• This phospho-specific antibody was raised against a synthetic peptide carrying phosphorylated Thr359 and Ser363. For Western blotting, confirm specificity by pre-incubating the antibody with the non-phosphorylated peptide or by treating samples with lambda phosphatase prior to immunoblotting.
• In WB, use mild or non-phosphate-containing blocking buffers (e.g., BSA or non-fat dry milk without phosphatase inhibitors) to preserve antigen recognition. Empirically optimize the primary antibody dilution (typically 1:500–1:2000) for your sample type and detection system.
• For ELISA, coat the phosphopeptide or protein extract and titrate the antibody to achieve an optimal signal-to-noise ratio. Consider using a non-phospho peptide as a negative control.
• Induce RPS6KA1 phosphorylation in human cell lines by treating with EGF (50 ng/mL) or insulin (100 nM) for 15–30 minutes prior to lysis, as RSK1 is strongly activated by the ERK pathway.
• Cross-reactivity has been demonstrated for human samples; for other species, verify sequence identity of the immunogen region before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material sourcing, offering validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents for immunoassay development. For researchers studying the MAP kinase signaling cascade, we supply phospho-specific and pan antibodies against key targets like RPS6KA1/RSK1. Let CamelBio be your partner in bridging from target discovery to diagnostic application, with technical consultation and reliable supply chains.