Phospho-p90Rsk/RSK1/RPS6KA1-T348 Rabbit pAb - Q15418

Core Product Specifications and Parameters

Biological Background: p90Rsk/RSK1/RPS6KA1 Function and Localization

• RPS6KA1 (p90RSK1) is a serine/threonine kinase acting downstream of ERK1/2 signaling, mediating mitogenic and stress-induced activation of transcription factors (CREB1, ETV1, NR4A1) and translational regulators (RPS6, EIF4B). Related references: PMID:10679322, PMID:12213813, PMID:15117958
• In fibroblasts, it is required for EGF-stimulated CREB1 phosphorylation and immediate-early gene expression. Related references: PMID:18508509, PMID:18813292
• The kinase modulates mTORC1 signaling by phosphorylating TSC2 (Ser-1798) and RPTOR, with feedback regulation through DEPTOR phosphorylation. Related references: PMID:15342917, PMID:22017876
• RPS6KA1 promotes cell survival by phosphorylating BAD and DAPK1, suppressing their pro-apoptotic functions. Related references: PMID:10679322, PMID:16213824
• It phosphorylates CDKN1B (p27Kip1), leading to its cytoplasmic retention and promoting G1/S cell cycle progression. Related references: PMID:18508509, PMID:18813292
• The kinase localizes to both the nucleus and cytoplasm, consistent with its dual roles in transcription factor activation and cytoplasmic signal transduction.
• During Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) infection, constitutively active RPS6KA1 drives viral lytic gene expression.
• UniProt keywords highlight that RPS6KA1 is an ATP-binding, magnesium-dependent kinase involved in the cell cycle, stress response, and host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic phosphorylated peptide around Thr348 of human RPS6KA1, ensuring specificity for the phosphorylated form at this activation site.
• For Western blot, consider optimizing the primary antibody dilution; an expected band near 83 kDa (predicted molecular weight) should be observed.
• This antibody cross-reacts with human, mouse, and rat samples; validation in your specific experimental system is recommended.
• When detecting phosphorylation-dependent signals, always include phosphatase inhibitors in lysis buffers to preserve in vivo phosphorylation states.
• The antibody is suitable for ELISA applications; explore its utility in capture or detection workflows after appropriate pairing validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop partner for IVD raw-material sourcing, supporting diagnostic manufacturers and research institutions with validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This anti-phospho-RPS6KA1 (T348) antibody addresses research into ERK/MAPK signaling, cell proliferation, and oncogenic processes. For your diagnostic or assay development requirements, reach out to CamelBio for expedited technical consultation and reliable supply solutions.