Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit Polyclonal Antibody for WB, IP, ELISA - Q15418

Core Product Specifications and Parameters

Biological Background: RPS6KA1 (p90Rsk/RSK1) Function and Localization

• Encodes the 90 kDa ribosomal protein S6 kinase alpha‑1 (p90‑RSK 1), also known as MAPKAP‑kinase 1a (MAPKAPK1a) and RSK‑1, a member of the AGC serine/threonine kinase family.
• Functions as a key downstream effector of the ERK/MAPK cascade, mediating mitogenic and stress‑induced signals that regulate transcription, translation, cell proliferation, survival, and differentiation. Related references: PMID:10679322, PMID:12213813, PMID:15117958
• Phosphorylates transcription factors CREB1, NR4A1/NUR77, and ETV1/ER81 upon EGF or PMA stimulation, driving immediate‑early gene expression and mitogenic responses. Related references: PMID:18508509, PMID:18813292, PMID:12213813, PMID:16223362
• Stimulates cap‑dependent translation by phosphorylating EIF4B and promotes pre‑initiation complex assembly through RPS6 phosphorylation. Related references: PMID:16763566, PMID:17360704
• Integrates nutrient and growth signals by directly phosphorylating TSC2 and RPTOR to modulate mTORC1 activity, and phosphorylates DEPTOR for feedback control of mTORC1/2. Related references: PMID:15342917, PMID:22017876
• Promotes cell survival by phosphorylating and inhibiting the pro‑apoptotic proteins BAD and DAPK1. Related references: PMID:10679322, PMID:16213824
• Regulates G1/S progression by phosphorylating CDKN1B/p27KIP1, leading to its cytoplasmic retention and prevention of nuclear cyclin‑dependent kinase inhibition. Related references: PMID:18508509, PMID:18813292
• Localizes to both the nucleus and cytoplasm, consistent with its dual roles in transcriptional regulation and cytoplasmic signaling.
• During Kaposi’s sarcoma‑associated herpesvirus/HHV‑8 infection, constitutively active RSK1 promotes viral lytic gene transcription and translation.
• Phosphorylation at Ser380, located in the hydrophobic motif, is essential for full kinase activation; this antibody specifically recognizes the phosphorylated Ser380 epitope.

Experimental Guidance and Technical Tips

• Since this antibody targets the phosphorylated Ser380 epitope, validate specificity by comparing stimulated versus unstimulated cell lysates (e.g., serum‑starved versus EGF‑ or PMA‑treated cells) and by treating lysates with lambda phosphatase.
• For Western blotting, prepare samples with phosphatase inhibitors and run appropriate molecular weight markers; the predicted band is approximately 83 kDa, though phosphorylation may cause a slight shift.
• Cross‑reactivity is reported for human, mouse, and rat; when testing other species, verify sequence homology around Ser380 in the target protein.
• Immunoprecipitation works under native conditions; consider using gentle lysis buffers that preserve protein‑protein interactions and phosphorylation status.
• For ELISA applications, use a phospho‑peptide coating antigen or capture the endogenous protein from cell extracts; assess linearity and background using non‑phosphorylated peptide controls.
• Store the antibody at –20 °C upon arrival and avoid repeated freeze‑thaw cycles; shipping on ice ensures stability.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides a streamlined, one‑stop sourcing solution for IVD raw materials, supporting diagnostic manufacturers, research laboratories, and clinical developers from early R&D through commercial scale‑up. With this Phospho‑RSK1 antibody, we address the growing demand for reliable, phospho‑specific reagents in cell signaling and kinase research. Beyond individual antibodies, our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal preparations, bulk ancillary reagents, and custom sourcing for rare or emerging targets — all backed by technical consultation to help you bridge the gap from concept to clinic.