Phospho-p90Rsk (T359/S363) Rabbit Monoclonal Antibody for WB - Q15418

Core Product Specifications and Parameters

Biological Background: p90Rsk (RPS6KA1) Function and Localization

• Protein identity: Ribosomal protein S6 kinase alpha-1, also known as p90-RSK 1, RSK-1, MAPKAPK1A, is encoded by the RPS6KA1 gene. It belongs to the RSK family of serine/threonine-protein kinases.
• Core signaling function: Acts downstream of ERK/MAPK signaling to mediate mitogenic and stress responses. It activates transcription factors CREB1, ETV1, and NR4A1, and regulates cell proliferation, survival, and differentiation via mTOR and apoptotic pathways. Related references: PMID:10679322, PMID:12213813, PMID:15117958
• Pro-survival role: Phosphorylates and inactivates pro-apoptotic proteins BAD and DAPK1, promoting cell survival. Related references: PMID:10679322, PMID:16213824
• mTOR pathway regulation: Directly phosphorylates TSC2 and RPTOR, enhancing mTORC1 activity, and also phosphorylates DEPTOR, providing feedback regulation of both mTORC1 and mTORC2. Related references: PMID:15342917, PMID:22017876
• Translational control: Phosphorylates RPS6 and EIF4B to promote translation initiation in response to growth factors and insulin. Related references: PMID:17360704, PMID:16763566, PMID:35772404
• Subcellular localization: Found in both nucleus and cytoplasm, consistent with its roles in transcriptional regulation and cytosolic signaling.
• Additional functions: Involved in cell cycle regulation by phosphorylating CDKN1B, and in cell migration via EPHA2 phosphorylation. Also implicated in hepatocellular proliferation and response to stress. Related references: PMID:18508509, PMID:18813292, PMID:26158630

Experimental Guidance and Technical Tips

• This antibody was generated using a synthetic phosphorylated peptide corresponding to residues around T359 and S363 of human RSK1. It is expected to specifically recognize the doubly phosphorylated form.
• For Western blot (WB), a band at approximately 83 kDa is anticipated. Use appropriate molecular weight markers.
• To preserve phosphorylation, include phosphatase inhibitors in lysis buffers. For negative control, consider phosphatase treatment of lysates.
• For immunoprecipitation (IP) and immunofluorescence (IF/ICC), optimize antibody concentration and fixation conditions for each experimental system.
• The antibody reacts with human, mouse, and rat targets, but sequence alignment of the phosphopeptide across these species is recommended for cross-reactivity confidence.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, technical services, and consulting, supporting manufacturers and labs from concept to clinic. We provide optimized monoclonal and polyclonal antibodies, validated antibody pairs, and bulk ancillary reagents for key signaling pathways such as ERK/MAPK. For specialized needs like phospho-specific RSK1 detection, we offer one-stop access to rare-target reagents and custom development.