Phospho-p70 S6 Kinase 1-T421/S424 Rabbit pAb for WB, ELISA - P23443

Core Product Specifications and Parameters

Biological Background: Ribosomal protein S6 kinase beta-1 Function and Localization

• Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression; regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Related references: PMID:11500364, PMID:12801526, PMID:14673156
• Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex; upon mitogenic stimulation, phosphorylation by mTORC1 leads to dissociation from the EIF3 complex and activation. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Related references: PMID:16286006, PMID:17053147
• Plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating MAPKAP1/SIN1, MTOR and RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling; also involved in feedback regulation of mTORC1 and mTORC2 by phosphorylating DEPTOR. Related references: PMID:15899889, PMID:19720745, PMID:19935711, PMID:22017876
• Mediates TNF-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. Related references: PMID:18952604
• Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR; phosphorylates EPRS and thereby plays a key role in fatty acid uptake by adipocytes and also most probably in interferon-gamma-induced translation inhibition. Related references: PMID:23429703, PMID:28178239
• Subcellular localization includes cytoplasm, nucleus, mitochondrion outer membrane, and synapse.
• Widely expressed in tissues.
• Contains multiple phosphorylation sites, acetylation, and alternative initiation; belongs to the AGC kinase family.

Experimental Guidance and Technical Tips

• Consider performing phosphatase treatment on lysates or using non‑phosphorylated peptide blocking to confirm the phospho‑specificity of the antibody.
• For Western blot, it is recommended to validate the antibody using positive control lysates from cells stimulated with growth factors or under nutrient‑rich conditions to induce p70 S6 Kinase 1 phosphorylation at T421/S424.
• The antibody is rabbit polyclonal; perform titration experiments to determine the optimal working dilution for your specific assay conditions.
• Cross‑reactivity has been observed with human, mouse, and rat samples; validate in the relevant species model system before large‑scale experiments.
• For ELISA‑based detection, ensure the capture/detection antibody pair distinguishes phosphorylated from non‑phosphorylated forms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting. This anti‑phospho‑p70 S6 Kinase 1 (T421/S424) antibody is part of our broad portfolio for mTOR signaling and cell growth research. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing to support your diagnostic development from concept to clinic.