Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb for WB, IF/ICC, ELISA - P23443

Core Product Specifications and Parameters

Biological Background: RPS6KB1 (p70 S6 Kinase 1) Function and Localization

• RPS6KB1 (p70 S6 kinase 1) is a serine/threonine-protein kinase that functions as a key downstream effector of the mTOR signaling pathway, regulating cell growth, proliferation, and survival. Related references: PMID:11500364, PMID:12801526, PMID:14673156
• The kinase is activated through multi-site phosphorylation, with mTORC1-mediated phosphorylation at Thr-421/Ser-424 (located in the autoinhibitory domain) playing a critical role in its activation. Upon mitogenic stimulation, this phosphorylation triggers dissociation from the EIF3 translation initiation complex and subsequent kinase activation. Related references: PMID:16286006
• It promotes protein synthesis by phosphorylating components of the translation machinery, including EIF4B, RPS6, and EEF2K, and enhances translation initiation by inactivating the EIF4A repressor PDCD4. Related references: PMID:11500364, PMID:12801526, PMID:14673156, PMID:17053147
• RPS6KB1 participates in feedback regulation of mTOR signaling by phosphorylating mTORC2 components MAPKAP1/SIN1 and RICTOR, as well as mTORC1 component DEPTOR, thereby modulating AKT1 activity. Related references: PMID:15899889, PMID:19720745, PMID:19935711, PMID:22017876
• It mediates cell survival by phosphorylating and inhibiting the pro-apoptotic protein BAD, and by regulating mitochondrial URI1-PPP1CC complex dynamics. Related references: PMID:17936702
• Subcellularly, it localizes to the cytoplasm, nucleus, mitochondria and mitochondrial outer membrane, and synaptic compartments, reflecting its diverse regulatory roles.
• The protein is widely expressed in human tissues, underscoring its fundamental role in cellular homeostasis.

Experimental Guidance and Technical Tips

• For Western blotting, a band of approximately 59 kDa is expected. Validate specificity using phosphatase-treated lysates or competition with non-phosphorylated peptide.
• In immunofluorescence, optimize fixation and permeabilization conditions to preserve the phospho-epitope; consider testing serum starvation and stimulation treatments to visualize activation-dependent localization changes.
• For ELISA applications, coat with the target phospho-peptide or use a sandwich format to capture and detect phospho-p70 S6K, ensuring the detection antibody recognizes the specific phosphorylation.
• When extending applications to mouse or rat samples, verify cross-reactivity in the relevant tissue or cell line, as sequence homology may influence binding.
• The immunogen design (synthetic phospho-peptide around T421/S424) ensures phospho-specificity; always confirm by treating samples with lambda phosphatase or using a non-phospho antibody control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for diagnostic manufacturers and research institutions, providing integrated access to IVD raw materials. For researchers investigating mTOR signaling and translation control, we offer a range of validated antibodies and ancillary reagents to support assay development. From optimized monoclonal and polyclonal antibodies to bulk ancillary reagents and rare-target sourcing, CamelBio streamlines procurement from concept to clinic.