Phospho-p70 S6 Kinase 1-T389 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P23443

Core Product Specifications and Parameters

Biological Background: p70 S6 Kinase 1 (S6K1) Function and Localization

• Encoded by RPS6KB1, p70 S6 kinase 1 (S6K1) is a serine/threonine kinase that acts downstream of mTORC1 to promote cell proliferation, growth, and cell cycle progression. Related references: PMID:11500364, PMID:12801526, PMID:14673156
• Phosphorylates key translation regulators including RPS6, EIF4B, EEF2K, PDCD4, and POLDIP3/SKAR, thereby enhancing global and pioneer rounds of protein synthesis. Related references: PMID:11500364, PMID:12801526, PMID:14673156, PMID:17053147, PMID:15341740
• In nutrient-depleted conditions, inactive S6K1 binds the EIF3 complex; mTORC1-dependent phosphorylation triggers its dissociation and activation, coupling nutrient signals to translation output. Related references: PMID:16286006
• Promotes cell survival through phosphorylation of BAD (suppressing apoptosis) and mitochondrial URI1, which initiates a negative feedback loop via PPP1CC-mediated dephosphorylation at Thr-412. Related references: PMID:11500364, PMID:12801526, PMID:14673156, PMID:17936702
• Phosphorylates mTORC2 components SIN1, MTOR, and RICTOR as well as DEPTOR, providing negative feedback that inhibits mTORC2 and AKT1 signaling. Related references: PMID:15899889, PMID:19720745, PMID:19935711, PMID:22017876
• Mediates TNF-induced insulin resistance via phosphorylation and degradation of IRS1, and activates CAD to drive pyrimidine biosynthesis downstream of mTOR. Related references: PMID:18952604, PMID:23429703
• Localizes to cytoplasm, nucleus, mitochondrial outer membrane, and synaptic compartments, reflecting its diverse cellular functions.
• Subject to extensive phosphorylation and acetylation; alternative initiation and splicing generate multiple isoforms; widely expressed across human tissues.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho-peptide spanning the Thr389 residue of human p70 S6K, a key mTORC1 target site; thus, the antibody is expected to specifically recognize the phosphorylated active form.
• For Western blotting, consider using phosphatase inhibitors during sample preparation to preserve the phosphorylation signal; a band around 59 kDa is anticipated.
• The antibody has been applied in ELISA, WB, and IF/ICC; we recommend titrating and validating in your experimental system.
• Cross-reactivity with mouse and rat is indicated, making it suitable for comparative mammalian studies.
• As a rabbit polyclonal antibody, it offers high sensitivity but may require careful blocking to reduce non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for high-quality IVD raw materials. This phospho-specific p70 S6K1 antibody supports research in cell signaling, oncology, and metabolism. In addition to primary antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your assay development.