Phospho-p70 S6 Kinase 1-T389 Rabbit pAb - P23443

Core Product Specifications and Parameters

Biological Background: Ribosomal protein S6 kinase beta-1 Function and Localization

• RPS6KB1 encodes the 70 kDa ribosomal protein S6 kinase beta-1 (p70S6K), also known as S6K1, p70 S6 kinase alpha, or serine/threonine-protein kinase 14A (STK14A).
• The kinase acts as a key downstream effector of mTORC1, regulating protein synthesis and cell growth. Upon mitogenic stimulation, mTORC1-mediated phosphorylation dissociates p70S6K from the EIF3 complex, enabling it to phosphorylate translation factors such as EIF4B and PDCD4, thereby promoting cap-dependent translation initiation. Related references: PMID:16286006 PMID:17053147
• p70S6K1 contributes to cell survival by inactivating the pro-apoptotic protein BAD through phosphorylation. Additionally, it phosphorylates mitochondrial URI1, disrupting the URI1-PPP1CC complex; released PPP1CC dephosphorylates p70S6K1 at Thr-412, creating a negative feedback loop for anti-apoptotic signaling. Related references: PMID:17936702
• The kinase exerts negative feedback on mTORC2/AKT1 signaling by phosphorylating components of mTORC2 (SIN1, MTOR, RICTOR), and also controls mTORC1 activity by phosphorylating DEPTOR. Related references: PMID:15899889 PMID:19720745 PMID:19935711 PMID:22017876
• p70S6K1 mediates TNF-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, targeting it for degradation. Related references: PMID:18952604
• It phosphorylates and activates CAD, the rate-limiting enzyme in de novo pyrimidine synthesis, thereby coupling mTOR signaling to nucleotide metabolism. Related references: PMID:23429703
• The protein localizes to multiple subcellular compartments including the cytoplasm, nucleus, mitochondrial outer membrane, and synapse/synaptosome. It is widely expressed and subject to alternative splicing and various post-translational modifications (e.g., phosphorylation, acetylation).

Experimental Guidance and Technical Tips

• For Western blotting, a band corresponding to the target protein is expected at approximately 59 kDa. Include phosphatase inhibitors in lysis buffers to preserve phosphorylation status.
• To confirm phospho-specificity, consider performing a dephosphorylation assay using lambda phosphatase or competing with the non-phosphorylated peptide.
• For IHC-P, optimize antigen retrieval conditions and antibody titration; inclusion of appropriate positive and negative controls is recommended.
• In ELISA, coat the plate with the phosphorylated immunogen peptide and validate signal linearity across a dilution series.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers, laboratories, and research institutes, offering one-stop access to critical raw materials from concept to clinic. This anti-phospho-p70 S6 Kinase 1 (T389) antibody supports research into mTOR signaling, oncology, and metabolic disorders. Beyond individual antibodies, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material procurement services to streamline your diagnostic development.