Phospho-p38 MAPK-T180 Rabbit pAb for WB, IHC-P, ELISA - Q16539

Core Product Specifications and Parameters

Biological Background: p38 MAPK Function and Localization

• MAPK14 encodes the p38 alpha mitogen-activated protein kinase, a serine/threonine kinase essential for cellular responses to stress, pro-inflammatory cytokines, and mitogens.
• It is one of four p38 MAPK family members (alpha, beta, gamma, delta) and is known by multiple aliases including CSBP, SAPK2A, and MXI2.
• Upon activation via dual phosphorylation at Thr-180 and Tyr-182, p38 alpha phosphorylates a broad range of substrates including downstream kinases MAPKAPK2/MK2, RPS6KA5/MSK1, and MKNK1/MNK1, thereby regulating gene expression, mRNA stability, and protein synthesis. Related references: PMID:9687510, PMID:9792677, PMID:11154262
• In the nucleus, activated p38 MAPK directly or indirectly phosphorylates transcription factors such as ATF1, ATF2, ELK1, p53/TP53, and MEF2C, modulating immediate-early gene expression and stress-induced transcriptional programs. Related references: PMID:10330143, PMID:9430721, PMID:9858528
• p38 MAPK also plays a cytoplasmic role in regulating protein turnover, endocytosis, and autophagy; for instance, it phosphorylates SIAH2 and modulates ATG9 trafficking. Related references: PMID:17003045, PMID:19893488
• Alternative splicing yields isoforms MXI2 (activated by mitogens/oxidative stress; weak kinase activity toward ELK1 and ATF2) and EXIP (pro-apoptotic).
• Subcellular fractionation reveals p38 MAPK in both cytoplasm and nucleus, consistent with its dual signaling and transcriptional roles.
• p38 MAPK is expressed in most tissues, with highest levels in brain, heart, placenta, pancreas, and skeletal muscle.

Experimental Guidance and Technical Tips

• Because the immunogen is a synthetic phosphorylated peptide around Thr-180 of human MAPK14, this antibody is expected to specifically recognize the phosphorylated, active form of p38 MAPK. For optimal detection, ensure that samples are prepared under conditions that preserve protein phosphorylation (e.g., use phosphatase inhibitors and avoid prolonged room-temperature incubations).
• For Western blot (WB), consider using a low-abundance protein enrichment method if endogenous p38 phosphorylation is weak; the predicted molecular weight is approximately 41 kDa.
• In immunohistochemistry (IHC-P), antigen retrieval and antibody dilution should be empirically determined; a phospho-specific signal can be validated by pre-treating tissue sections with lambda phosphatase.
• For ELISA applications, use a recombinant phosphorylated p38 standard where possible, and confirm linearity and specificity using non-phosphorylated peptide controls.
• Cross-reactivity has been observed with human, mouse, and rat, but validation in your specific experimental system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio offers a comprehensive range of IVD raw materials, validated antibody pairs, and ancillary reagents to support diagnostic assay development from target discovery to clinical validation. Whether you need bulk quantities of this anti-phospho-p38 (T180) polyclonal antibody or matched pairs for sandwich ELISA, our one-stop sourcing model simplifies procurement, saving time and resources. Our expertise extends to rare and niche targets, ensuring that researchers and manufacturers can access the critical raw materials required for MAPK pathway studies and inflammatory disease diagnostics.