Phospho-NMDAR1-S896 Rabbit Polyclonal Antibody for WB, ELISA - Q05586

Core Product Specifications and Parameters

Biological Background: Glutamate Receptor Ionotropic NMDA 1 (GRIN1) Function and Localization

• The NMDA receptor is a heterotetrameric ligand-gated cation channel composed of GluN1, GluN2, and GluN3 subunits. The GluN1 subunit (encoded by GRIN1) is essential for channel function, binding glycine or D-serine as a co-agonist.
  Related references: PMID:21376300, PMID:26875626, PMID:26919761
• Channel activation requires simultaneous binding of glutamate to GluN2 subunits and glycine/D-serine to GluN1, along with membrane depolarization to relieve voltage-dependent Mg²⁺ block. This allows influx of Ca²⁺ and Na⁺ and efflux of K⁺.
  Related references: PMID:21376300, PMID:26875626, PMID:26919761
• NMDARs play a critical role in synaptic plasticity, long-term potentiation (LTP), learning, and memory formation.
  Related references: PMID:26875626
• Different GluN2 and GluN3 subunits confer distinct kinetic and pharmacological properties to the receptor, including sensitivity to pH, allosteric modulators, and Ca²⁺ permeability.
  Related references: PMID:26875626, PMID:26919761, PMID:36309015
• The GluN1 subunit is localized to the postsynaptic density membrane, synaptic cell membrane, and cell membrane, consistent with its role in excitatory neurotransmission.
• GRIN1 undergoes extensive post-translational modification, including phosphorylation, glycosylation, disulfide bond formation, and ubiquitination. Phosphorylation at serine 896 (among other sites) regulates receptor trafficking and function.
• Mutations in GRIN1 are linked to neurological diseases such as epilepsy and intellectual disability, highlighting its importance in normal brain function.

Experimental Guidance and Technical Tips

• For Western blot, the recommended band is expected around 105 kDa. Consider using phospho‑specific controls, such as phosphatase‑treated lysates, to confirm signal specificity.
• In ELISA, pair the antibody with non‑phosphorylated peptide or protein to assess phosphorylation‑dependent reactivity. Validate assay conditions using your own experimental system.
• As a polyclonal antibody, lot‑to‑lot variability may occur. Always titrate and optimize dilution factors for each new lot in your specific sample matrix.
• Cross‑reactivity with human, mouse, and rat is predicted based on sequence homology around the immunogen site, but verification in target species is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined supply channel for IVD raw materials. In the field of neuroscience and NMDA receptor studies, we can source validated phospho‑specific antibodies, recombinant proteins, and ancillary reagents tailored to your assay development and scale‑up needs. Our team also supports custom procurement of rare targets and bulk materials, helping you bridge the gap from concept to clinic efficiently.