Phospho-NFKB1-S927 Rabbit pAb for WB, ELISA - P19838

Core Product Specifications and Parameters

Biological Background: NFKB1 Function and Localization

• NFKB1 encodes the p105 precursor which is processed into the active p50 subunit of NF-kappa-B. P105 acts as a cytoplasmic retention factor for attached NF-kappa-B proteins. Related references: PMID:1423592
• The active p50 subunit associates with RELA/p65 to form the NF-kappa-B p65-p50 complex, a transcription factor that binds kappa-B sites in DNA. Related references: PMID:1740106 PMID:7830764
• NF-kappa-B is a pleiotropic transcription factor involved in inflammation, immunity, cell growth, and apoptosis, existing as various dimers with distinct transcriptional activities.
• The p50-p50 homodimer acts as a transcriptional repressor, but can become an activator when associated with BCL3.
• Subcellular location is primarily cytoplasmic; upon activation, NF-kappa-B complexes translocate to the nucleus.
• Key post-translational modifications include acetylation, phosphorylation, and ubiquitination, regulating its activity.
• Serine 927 phosphorylation may modulate NFKB1 function; the antibody is specific for this modification.
• Gene family: NFKB1 is a member of the NF-kappa-B family, which includes RELA, RELB, REL, and NFKB2.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to serine 927 of human NFKB1. For Western blot, consider using appropriate phosphatase inhibitors during sample preparation to preserve the phosphorylation.
• The antibody is generated in rabbit and is expected to be specific for the phosphorylated form; it may also recognize the non-phosphorylated form if present abundantly. Validate in relevant positive and negative control lysates.
• For ELISA, the peptide can be used as a coating antigen or competitor to confirm specificity.
• Since the protein weight is 105 kDa, ensure adequate gel percentage for separation (e.g., 8–10% SDS-PAGE) and use appropriate molecular weight markers.
• Cross-reactivity is reported for human; if using in other species, sequence alignment around S927 is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This Phospho-NFKB1-S927 antibody is part of our extensive portfolio for NF-kappa-B pathway research, supporting applications such as Western blot and ELISA. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance with sourcing rare targets to accelerate your assay development.