Phospho-NFKB1 (S337) Rabbit Polyclonal Antibody for WB, ELISA - P19838

Core Product Specifications and Parameters

Biological Background: NFKB1 Function and Localization

• NFKB1 encodes the p105 precursor, which undergoes co‑ and post‑translational proteasome‑mediated processing to generate the active p50 subunit. p50 forms homo‑ or heterodimers with other NF‑κB family members (RELA, RELB, REL, NFKB2). Related references: PMID:1423592
• The p65‑p50 heterodimer is the most abundant NF‑κB complex and acts as a transcriptional activator by binding to κB consensus sites (5′‑GGRNNYYCC‑3′) in the enhancers of immune and acute‑phase response genes. Related references: PMID:1740106 PMID:7830764
• The p50‑p50 homodimer functions as a transcriptional repressor but can switch to an activator when associated with BCL3. NFKB1 p105 also retains NF‑κB proteins in the cytoplasm, thereby controlling their nuclear translocation.
• NF‑κB signaling is tightly regulated by post‑translational modifications, including phosphorylation, acetylation, S‑nitrosylation, and ubiquitination. NFKB1 is a phosphoprotein; phosphorylation events modulate its stability, processing, and transcriptional activity.
• NFKB1 is widely expressed, particularly in immune cells, and plays a central role in inflammation, immunity, apoptosis, and cell proliferation.
• The protein localizes primarily to the cytoplasm in resting cells. Upon activation and degradation of IκB inhibitors, the freed dimers translocate to the nucleus to induce target gene expression.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around S337 of human NFKB1. To preserve the phosphorylation signal during sample preparation, include phosphatase inhibitors in lysis buffers and avoid prolonged incubations at elevated temperatures.
• For Western blot, expect major bands at ~50 kDa (p50 subunit) and ~105 kDa (p105 precursor). Due to cross‑reactivity with mouse and rat orthologs, it can be used in these species, but optimal dilution should be determined empirically.
• The antibody has been validated for WB and ELISA. When performing ELISA, consider a sandwich format or a competitive assay design, and verify specificity using non‑phosphorylated peptide controls.
• NF‑κB activation can be induced by a wide array of stimuli (e.g., TNF‑α, LPS, IL‑1β). Whenever possible, include positive controls from stimulated cells and a dephosphorylated or phosphatase‑treated sample to confirm phosphorylation dependence.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with reliable one‑stop access to IVD raw materials. This Phospho‑NFKB1‑S337 antibody supports investigations into NF‑κB signaling, inflammation, and related pathologies. Beyond single reagents, CamelBio can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized raw‑material sourcing for rare or niche targets—streamlining your journey from concept to clinic.