Phospho-NF2-S518 Rabbit pAb - P35240

Core Product Specifications and Parameters

Biological Background: NF2 Function and Localization

• NF2 encodes Merlin (Moesin-ezrin-radixin-like protein), also known as Schwannomin, a member of the ERM (ezrin/radixin/moesin) protein family that links actin cytoskeleton to membrane proteins.
• Merlin functions as a tumor suppressor by regulating the Hippo/SWH signaling pathway; it promotes phosphorylation of LATS1/2 kinases to restrict cell proliferation and induce apoptosis.
• It may stabilize the cell membrane and inhibit PI3 kinase signaling by binding to AGAP2 and impairing its stimulatory activity.
• Merlin suppresses tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex, preventing degradation of pro-apoptotic factors.
• Subcellularly, Merlin localizes to the plasma membrane at filopodia and ruffles, as well as the cytoplasm, perinuclear region, cytoplasmic granules, nucleus, and cytoskeleton, reflecting dynamic roles in contact inhibition.
• The protein is widely expressed; multiple isoforms exist, with isoform 1 and 3 predominating, and others showing tissue-specific patterns (e.g., retina, fetal tissues).
• Key post-translational modification includes phosphorylation at Ser518, which modulates its conformation and tumor suppressor activity, as reflected by the UniProt keyword "phosphoprotein".
• Merlin is associated with the disease neurofibromatosis type 2 (NF2), and mutations in this gene cause deafness; it is listed as a reference proteome member and tumor suppressor.

Experimental Guidance and Technical Tips

• This antibody was raised against a phosphorylated peptide around serine 518; it is expected to specifically recognize the phosphorylated form of NF2/Merlin. Include phosphatase inhibitors in lysis buffers to preserve phosphorylation.
• For western blotting, a band at approximately 70 kDa should be observed. To confirm phospho-specificity, consider treating samples with lambda phosphatase or using a non-phospho peptide competition control.
• In immunohistochemistry (IHC-P), validate the antibody in tissue sections known to express phosphorylated Merlin, such as human schwannoma or normal brain tissues, and include an isotype control.
• ELISA applications can be performed using the phosphorylated peptide as coating antigen; a non-phosphorylated peptide can serve as a negative control.
• Cross-reactivity with mouse and rat is reported; however, due to sequence conservation, verify performance in each species' relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined supply channel for IVD raw materials, including this phospho-specific NF2-S518 antibody ideal for tumor suppressor and Hippo pathway studies. As a B2B sourcing partner, we provide validated antibody pairs, bulk monoclonal and polyclonal antibodies, and ancillary reagents to support your diagnostic assay development. For rare target or custom phospho-antibody needs, our technical team can assist in sourcing or development to move your project from concept to clinic.