Phospho-NF-κB p65/RelA (Ser468) Rabbit Polyclonal Antibody for WB, ELISA - Q04206

Core Product Specifications and Parameters

Biological Background: NF-κB p65/RelA Function and Localization

• NF-κB p65 (also known as RelA, NFKB3) is a subunit of the NF-κB transcription factor complex, belonging to the Rel homology domain-containing protein family that includes RELA, RELB, REL, NFKB1/p105, and NFKB2/p52.
• The canonical NF-κB heterodimer RELA-NFKB1 binds κB DNA sites to activate genes involved in inflammation, immunity, cell growth, differentiation, and apoptosis.
• p65/RelA forms both activating heterodimers (e.g., RELA-NFKB1) and homodimers (RELA-RELA), the latter involved in invasin-mediated IL-8 expression.
• Subcellular localization is primarily cytoplasmic in an inactive state bound to IκB inhibitors; upon activation, it translocates to the nucleus to regulate transcription.
• p65 is subject to extensive post-translational modifications including phosphorylation, acetylation, methylation, ubiquitination, and S-nitrosylation, which modulate its activity and stability.
• Phosphorylation at Ser468 (and other residues) is implicated in fine-tuning transcriptional activity and protein interactions.
• RelA is essential for T-cell cytokine gene expression. Related references: PMID:15790681
• Acts as a key transcription factor in the interferon response during SARS-CoV-2 infection. Related references: PMID:33440148

Experimental Guidance and Technical Tips

• This antibody is raised against a synthetic phosphopeptide around Ser468; therefore, it specifically recognizes the phosphorylated form of p65/RelA. For accurate detection, include phosphatase inhibitors in lysis and wash buffers.
• To confirm phospho-specificity, treat samples with lambda protein phosphatase and compare band signals by WB; a loss of signal indicates phosphorylation dependence.
• For WB, perform SDS-PAGE under reducing conditions and use appropriate controls (e.g., untreated vs. stimulated cell lysates known to activate NF-κB).
• For ELISA, the antibody may be used as a capture or detection reagent; validate pairing with a non-phospho-specific p65 antibody for sandwich ELISA.
• Consider cross-reactivity with rat samples as indicated; however, validate in your specific experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw-material sourcing, providing validated phospho-specific antibodies like this NF-κB p65/RelA product to support research in inflammation and signal transduction. Our portfolio spans optimized antibody pairs, bulk reagents, and rare-target sourcing, empowering diagnostic developers and researchers to streamline assay development from concept to clinic.