Phospho-NEDD4L-S448 Rabbit pAb for WB, ELISA - Q96PU5

Core Product Specifications and Parameters

Biological Background: NEDD4L Function and Localization

• E3 ubiquitin-protein ligase that mediates polyubiquitination of target proteins, regulating signaling pathways including autophagy, innate immunity, and DNA repair. Related references: PMID:20064473, PMID:31959741, PMID:33608556
• Inhibits TGF-beta signaling by promoting ubiquitination and proteasome-dependent degradation of SMAD2 and TGFBR1. Related references: PMID:15496141
• Downregulates autophagy and cell growth by ubiquitinating and reducing cellular levels of ULK1 and ASCT2. Related references: PMID:28820317, PMID:31959741
• Ubiquitinates and induces internalization of various plasma membrane ion channels, including ENaC, voltage-gated sodium channels (Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8), potassium channels (Kv1.3, KCNH2, KCNQ2/3), and CLC5. Related references: PMID:26363003, PMID:27445338
• Ubiquitinates and regulates protein stability of signaling molecules such as SGK1, TNK2, BRAT1, NTRK1, TTYH2, and TTYH3. Related references: PMID:25631046, PMID:27445338, PMID:18577513
• Involved in dendrite formation by melanocytes and regulation of TOR signaling. Related references: PMID:23999003, PMID:27694961
• Subcellular localization includes the cytoplasm, Golgi apparatus, and endosomal multivesicular bodies.
• Ubiquitously expressed, with highest levels in prostate, pancreas, and kidney; also expressed in melanocytes. Related references: PMID:14615060, PMID:15496141, PMID:19664597, PMID:23999003
• Phosphorylated at multiple residues including Ser-448; also acetylated and undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The antibody was generated using a synthetic phosphorylated peptide around Ser-448 of NEDD4L, so it is expected to specifically recognize the phosphorylated form of the protein.
• For Western blotting, validate the ~112 kDa band in appropriate cell/tissue lysates; consider using phosphatase inhibitors and stimulation conditions that modulate NEDD4L phosphorylation.
• In ELISA applications, optimize coating and blocking conditions to ensure specificity for the phosphorylated epitope, and include non-phosphorylated peptide controls to confirm phospho-selectivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined procurement experience for IVD raw materials, supporting research and diagnostic development from concept to clinic. This anti-Phospho-NEDD4L-S448 antibody is part of an extensive catalog of validated antibodies, proteins, and ancillary reagents for studying ubiquitin ligases and phosphorylation-dependent signaling. For bulk orders, custom sourcing, or technical consultation, our team facilitates rapid access to the critical materials you need.