Phospho-Na+/K+-ATPase-S16 Rabbit pAb for WB - P05023

Core Product Specifications and Parameters

Biological Background: ATP1A1 (Na+/K+-ATPase subunit alpha-1) Function and Localization

• Catalyzes the active transport of Na+ out of and K+ into the cell, establishing electrochemical gradients critical for membrane potential, nutrient uptake, and cell volume control. Related references: PMID:29499166, PMID:30388404, PMID:35803952
• Undergoes E1/E2 conformational transitions powered by ATP hydrolysis, alternating ion binding and release to drive 3 Na+ out / 2 K+ in per cycle. Related references: PMID:35803952
• Member of the P-type ATPase family with ten transmembrane domains and intracellular ATP-binding and phosphorylation sites; the alpha-1 subunit is the most ubiquitously expressed isoform.
• Localized primarily at the basolateral membrane in epithelia, sarcolemma of muscle, axonal projections, and melanosomes, matching its roles in transepithelial transport and excitability.
• Phosphorylation at Ser16 (as detected by this antibody) is a regulatory modification; the protein is also acetylated and undergoes alternative splicing affecting its function.
• Mutations in ATP1A1 are linked to Charcot-Marie-Tooth disease type 2DD, hypomagnesemia, epilepsy, and intellectual disability, underscoring its importance in the nervous system.

Experimental Guidance and Technical Tips

• Raised against a phosphorylated peptide around Ser16, this antibody is expected to recognize the endogenous protein only when phosphorylated at this site. Validate specificity by comparing signals from phosphatase-treated and untreated samples.
• For Western blot, prepare lysates with phosphatase inhibitors and consider using a non-phospho blocking peptide to confirm band identity.
• In ELISA, use the immunogen phospho-peptide and a non-phospho counterpart to assess cross-reactivity.
• The antibody cross-reacts with human, mouse, and rat; titrate for each species as expression levels may differ.
• As a polyclonal antibody, lot-to-lot variation can occur; re-validate in your assay when receiving a new lot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing bridge for IVD raw materials, offering everything from validated antibody pairs and optimized polyclonal/monoclonal antibodies to bulk ancillary reagents and custom rare-target sourcing. This phospho-specific ATP1A1 antibody exemplifies our commitment to supporting ion transport and neurology-focused research and diagnostic development.