Phospho-Myosin Light Chain 2-T18/S19 Rabbit mAb - P24844

Core Product Specifications and Parameters

Biological Background: Myosin Light Chain 2 Function and Localization

• Encodes myosin regulatory light polypeptide 9 (MYL9), also known as LC20, MLC-2C, or MRLC1; alternative splicing generates multiple isoforms.
• Plays a key role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation, and is implicated in cytokinesis, receptor capping, and cell locomotion (PubMed:11942626, PubMed:2526655). Related references: PMID:11942626 PMID:2526655
• In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation (By similarity).
• Localizes to the cytoplasm, cytoskeleton, and cell cortex.
• Expressed in smooth muscle tissues and in some, but not all, nonmuscle cells.
• Subject to critical post-translational modifications including phosphorylation (e.g., at T18/S19), acetylation, and calcium/metal binding.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide spanning T18/S19; verify phosphorylation specificity by comparing signals in native versus phosphatase-treated lysates.
• Suitable for Western blot (predicted band at ~20 kDa) and ELISA; consider using known phospho-myosin regulators as controls.
• Validate antibody performance in human smooth muscle or nonmuscle cell models relevant to your study.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your diagnostic and research needs. For myosin light chain 2 phosphorylation studies in cell contractility and smooth muscle biology, we provide end-to-end procurement and technical support to accelerate your projects from concept to clinic. Our supply network can source rare-target reagents to complement this phospho-MLC2 antibody, ensuring a reliable one-stop bridge to development-ready materials.