Phospho-Myosin Light Chain 2-T18 Rabbit mAb - P24844

Core Product Specifications and Parameters

Biological Background: MYL9 / Myosin Light Chain 2 Function and Localization

• MYL9 encodes myosin regulatory light polypeptide 9, also known as 20 kDa myosin light chain (LC20), MLC-2C, myosin regulatory light chain 2 smooth muscle isoform, myosin regulatory light chain MRLC1.
• Functions as a myosin regulatory subunit crucial for regulating both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (PubMed:11942626, PubMed:2526655). Related references: PMID:11942626 PMID:2526655
• May regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation in myoblasts (By similarity).
• Localizes to the cytoplasm, cytoskeleton, and cell cortex.
• Expressed in smooth muscle tissues and also in some, but not all, nonmuscle cells.
• Contains calcium-binding EF-hand motifs and is subject to regulatory phosphorylation; classified as a metal-binding, actin-associated motor protein.
• Alternative splicing yields multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to phosphorylated T18 of human MYL9. To preserve the phosphorylation signal, we recommend using phosphatase inhibitors in lysis buffers and performing rapid sample processing.
• For Western blotting, the predicted molecular weight is approximately 20 kDa; optimize blocking and antibody incubation conditions for phospho-specific detection.
• In ELISA, consider using a non-phosphorylated control peptide to confirm phosphorylation-specific binding.
• Validate the antibody in your relevant cell/tissue system under conditions that modulate MYL9 phosphorylation (e.g., serum starvation vs. stimulation of smooth muscle cells).
• Since the antibody is validated for human, cross-reactivity in other species should be experimentally determined if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For research on smooth muscle contractility and myosin light chain regulation, we supply validated phospho-specific antibodies, optimized monoclonal/polyclonal antibody pairs, and bulk ancillary reagents to support your assay development. Our global sourcing network also facilitates procurement of rare-target raw materials, streamlining your path from research to commercialization.