Phospho-MYL9-T18/S19 Rabbit pAb for WB, IHC-P, ELISA - P24844

Core Product Specifications and Parameters

Biological Background: Myosin Regulatory Light Polypeptide 9 (MYL9) Function and Localization

• MYL9 encodes the myosin regulatory light chain 9, a 20 kDa protein also known as LC20, MLC-2C, or MRLC1, serving as a regulatory subunit of myosin II.
• It regulates both smooth muscle and nonmuscle cell contractile activity via phosphorylation, playing essential roles in cytokinesis, receptor capping, and cell locomotion. Related references: PMID:11942626 PMID:2526655
• In myoblasts, MYL9 may modulate PIEZO1-dependent cortical actomyosin assembly important for myotube formation (By similarity).
• Subcellularly, the protein localizes to the cytoplasm, cytoskeleton, and cell cortex, consistent with its contractile functions.
• It is predominantly expressed in smooth muscle tissues, with selective presence in certain nonmuscle cells.
• Post-translational modifications include acetylation and extensive phosphorylation, which are critical for its regulatory activity.
• The protein features calcium-binding EF-hand motifs and belongs to the myosin regulatory light chain family, with alternative splicing generating multiple isoforms.

Experimental Guidance and Technical Tips

• This antibody was raised against a phosphopeptide covering Thr18 and Ser19 of human MYL9. Phospho-specific detection may be enhanced by using BSA-based blocking buffers free of phosphate-containing additives.
• In Western blotting, anticipate a band near 20 kDa. Confirm phospho-specificity by treating cell or tissue lysates with lambda phosphatase to observe signal reduction or mobility shift.
• For IHC-P, antigen retrieval methods (e.g., heat-induced citrate buffer, pH 6.0) may improve staining. Employ smooth muscle-rich tissues such as aorta or uterus as positive controls.
• In ELISA applications, this polyclonal antibody can be employed for phospho-peptide capture or detection; pairing with a total MYL9 antibody allows normalization of phosphorylation levels.

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CamelBio serves as a dedicated one-stop partner for IVD raw material procurement, providing access to premium reagents like this phospho-MYL9(T18/S19) polyclonal antibody for cytoskeleton and contractility studies. Our portfolio encompasses validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, alongside specialized sourcing for rare targets to support every stage of your diagnostic development.