Phospho-MYL9 (Ser19) Rabbit Polyclonal Antibody for WB and ELISA - P24844

Core Product Specifications and Parameters

Biological Background: MYL9 Function and Localization

• MYL9 encodes myosin regulatory light polypeptide 9, also known as 20 kDa myosin light chain (LC20), MLC-2C, and myosin regulatory light chain MRLC1.
• It functions as a regulatory subunit of myosin, playing a key role in smooth muscle and non-muscle cell contractile activity via its phosphorylation. Related references: PMID:11942626 PMID:2526655
• MYL9 is implicated in diverse cellular processes including cytokinesis, receptor capping, cell locomotion, and cortical actomyosin assembly during myotube formation. Related references: PMID:11942626 PMID:2526655
• The protein localizes to the cytoplasm, cytoskeleton, and cell cortex, consistent with its contractile functions.
• Tissue expression is prominent in smooth muscle tissues and selected non-muscle cells.
• Key post-translational modifications include phosphorylation (as targeted by this antibody), acetylation, and calcium binding, influencing its regulatory role.
• Alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• To confirm phosphorylation specificity in western blot, consider treating samples with lambda phosphatase or performing peptide competition assays with non-phosphorylated S19 peptide.
• For ELISA, the phosphorylated immunogen peptide can serve as a coating antigen to detect phospho-specific MYL9 signals; validate in the relevant sample system.
• Given cross-reactivity with human, mouse, and rat, use appropriate positive lysates (e.g., smooth muscle or non-muscle cells) and monitor for expected 20 kDa band.
• As a polyclonal antibody, lot-to-lot consistency should be verified; always run positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers streamlined access to validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents for smooth muscle and contractile function research. Whether you need this phospho-MYL9 antibody or other rare-target raw materials, our one-stop sourcing ensures consistent supply from development to clinical application. Partner with us to accelerate your IVD pipeline.