Phospho-MYH9-S1943 Rabbit pAb - P35579

Core Product Specifications and Parameters

Biological Background: Myosin-9 Function and Localization

• Myosin-9 (MYH9) is a non-muscle myosin heavy chain IIA (NMMHC-IIA), a motor protein that drives actin-based motility.
• It is essential for cytokinesis, cell shape maintenance, and specialized functions such as secretion and capping.
• Myosin-9 promotes cell motility in complex with S100A4. Related references: PMID:16707441
• During cell spreading, it contributes to cytoskeletal reorganization, focal contact formation, and lamellipodial retraction. Related references: PMID:20052411
• It localizes to the cytoplasm, cytoskeleton, cell cortex, and specific membrane regions, including secretory vesicles and the cell membrane.
• It undergoes various post‑translational modifications including acetylation, ubiquitination, and phosphorylation (e.g., at S1943), which regulate its activity.
• It is expressed in kidney glomeruli and leukocytes, and mutations are associated with diseases like Alport syndrome and deafness.
• Additionally, myosin-9 serves as a receptor for herpes simplex virus 1 (HSV-1) infection.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around serine 1943 of human MYH9; verify phosphorylation‑dependent detection using phosphatase‑treated samples in WB.
• For WB, the expected band size is approximately 227 kDa; consider using a low‑percentage gel and extended transfer times.
• ELISA may be used to quantify phosphorylation levels; include non‑phosphorylated peptide controls to confirm specificity.
• Cross‑reactivity with mouse and rat indicates conserved sequences; validate specificity in the relevant sample system.

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