Phospho-mTOR-S2448 Rabbit mAb for WB, ELISA - P42345

Core Product Specifications and Parameters

Biological Background: mTOR Function and Localization

• MTOR encodes a serine/threonine-protein kinase that serves as a central regulator of cellular metabolism, growth, and survival, integrating signals from hormones, growth factors, nutrients, and stress (PubMed:12087098, PubMed:12150925, PubMed:12150926). Related references: PMID:12087098 PMID:12150925 PMID:12150926
• As part of the mTORC1 complex, it promotes anabolic processes such as protein synthesis via phosphorylation of EIF4EBP1 and RPS6KB1, lipid and nucleotide synthesis, and simultaneously inhibits catabolic pathways including autophagy through ULK1 phosphorylation (PubMed:12087098, PubMed:12150925, PubMed:12150926, PubMed:24403073, PubMed:29236692, PubMed:18925875, PubMed:23429703, PubMed:23429704, PubMed:20516213, PubMed:23426360, PubMed:32561715, PubMed:20537536, PubMed:30704899, PubMed:23524951, PubMed:25438055, PubMed:21659604, PubMed:12231510, PubMed:22343943, PubMed:22576015, PubMed:22692423, PubMed:34289345). Related references: PMID:12087098 PMID:12150925 PMID:12150926 PMID:24403073 PMID:29236692 PMID:18925875 PMID:23429703 PMID:23429704 PMID:20516213 PMID:23426360 PMID:32561715 PMID:20537536 PMID:30704899 PMID:23524951 PMID:25438055 PMID:21659604 PMID:12231510 PMID:22343943 PMID:22576015 PMID:22692423 PMID:34289345
• In the mTORC2 complex, it regulates cell proliferation, survival, and cytoskeletal organization by phosphorylating AGC kinases including AKT (at Ser473), PKC, and SGK1 (PubMed:15268862, PubMed:15467718, PubMed:24670654, PubMed:21376236, PubMed:15718470, PubMed:18925875). Related references: PMID:15268862 PMID:15467718 PMID:24670654 PMID:21376236 PMID:15718470 PMID:18925875
• mTOR localizes to various cellular membranes including lysosome, endoplasmic reticulum, Golgi, and mitochondria, as well as the cytoplasm and nucleus.
• It is broadly expressed with highest levels in testis, and is subject to post-translational modifications such as phosphorylation, acetylation, and ubiquitination.

Experimental Guidance and Technical Tips

• Include phosphatase inhibitors in lysis buffers to preserve phosphorylation signals during sample preparation for WB or ELISA.
• Validate antibody specificity using positive (e.g., growth factor-stimulated) and negative (e.g., phosphatase-treated or serum-starved) control lysates.
• For WB, use an appropriate gel percentage and transfer conditions for the high molecular weight target (289 kDa); consider a 6-8% resolving gel.
• In ELISA, compare binding to phosphorylated versus non-phosphorylated peptide antigens to confirm phospho-specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, supporting your diagnostic and research needs from concept to clinic. This phospho-mTOR (Ser2448) rabbit monoclonal antibody is part of our growing catalog for cellular signaling and oncology research. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary materials, and specialized sourcing for rare targets—streamlining your procurement process.