Phospho-MRE11 (Ser676) Rabbit pAb for WB - P49959

Core Product Specifications and Parameters

Biological Background: MRE11 Function and Localization

• MRE11 is the core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, and meiosis.
  Related references: PMID:11741547 PMID:14657032 PMID:22078559

• Possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, initiating DNA end resection after DSBs.
  Related references: PMID:11741547 PMID:22078559 PMID:24316220

• Endonuclease activity cleaves the 5' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence homologous recombination (HR); subsequent exonucleolytic degradation creates a single-stranded DNA gap for EXO1- and DNA2-mediated long-range resection.
  Related references: PMID:24316220 PMID:28867292

• Cooperates with RBBP8/CtIP, which specifically promotes the endonuclease activity of MRE11 to clear protein-DNA adducts and generate clean double-strand break ends.
  Related references: PMID:27814491 PMID:27889449 PMID:30787182

• The MRN complex is required for DNA damage signaling via activation of the ATM and ATR kinases; the nuclease activity of MRE11 is not required for this function.
  Related references: PMID:14657032 PMID:15064416 PMID:15790808

• Involved in the processing of R-loops and, by similarity, activation of the cGAS-STING pathway by displacing CGAS from nucleosome sequestration.
  Related references: PMID:31537797

• Localizes to the nucleus, chromosome, and telomeres, consistent with its role in DNA repair and telomere maintenance.

• As a phosphoprotein, MRE11 is subject to phosphorylation (including at Ser676), acetylation, methylation, and ubiquitin-like conjugation, potentially regulating its functions.

Experimental Guidance and Technical Tips

• For phospho-specific detection, include phosphatase inhibitors in lysis buffer and verify detection by treating samples with phosphatase or using non-phosphorylated competitors.
• The predicted molecular weight is ~81 kDa; run a suitable SDS-PAGE gel percentage (e.g., 8-10%) and include a molecular weight marker for accurate band identification.
• As a polyclonal antibody, consider validating specificity and consistency for each new lot in your experimental system.
• Confirm reactivity in DNA damage models (e.g., ionizing radiation or genotoxic agent treatment) to enrich for phosphorylated MRE11 at Ser676.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, from concept to clinic. This anti-phospho-MRE11 (Ser676) antibody supports DNA damage and repair research, and we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Contact us to discuss your procurement needs for diagnostic development.