Phospho-MLKL-S358 Rabbit pAb - Q8NB16

Core Product Specifications and Parameters

Biological Background: MLKL Function and Localization

• MLKL (Mixed lineage kinase domain-like protein) is a pseudokinase that lacks intrinsic kinase activity and serves as a critical executioner in the necroptosis pathway. Related references: PMID:22265413 PMID:22265414 PMID:22421439
• Upon phosphorylation by RIPK3 at S358, MLKL homotrimerizes, translocates to the plasma membrane, and triggers necrotic cell death via calcium influx and membrane rupture. Related references: PMID:22265413 PMID:22265414 PMID:22421439
• In response to orthomyxovirus infection, nuclear RIPK3 phosphorylates MLKL, leading to nuclear envelope disruption and leakage of cellular DNA into the cytosol (by similarity).
• MLKL binds to inositol hexakisphosphate (InsP6), which is essential for its necroptotic function. Related references: PMID:29883610
• Subcellular localization includes cytoplasm, cell membrane, and nucleus, consistent with dynamic translocation during necroptosis.
• MLKL is a phosphoprotein; alternative splicing, coiled-coil, and nucleotide-binding domains are noted.
• The predicted molecular weight of human MLKL is approximately 54 kDa (UniProt: 54479 Da).

Experimental Guidance and Technical Tips

• For Western blot, use phosphatase inhibitors during sample preparation to preserve the phosphorylated S358 epitope.
• Include a non-phosphorylated control or phosphatase treatment to confirm phospho-specificity.
• The antibody cross-reacts with rat MLKL; validate in the appropriate tissue or cell system if using rat samples.
• In ELISA, the synthetic phospho-peptide immunogen can serve as a positive control for assay development.
• A band of approximately 54 kDa is expected for human MLKL in Western blot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and researchers to provide a one-stop platform for IVD raw materials, from concept to clinic. This phospho-MLKL-S358 antibody supports necroptosis and cell death pathway research, and we can supply additional validated antibodies, bulk reagents, and rare-target raw materials tailored to your workflow. Let CamelBio simplify your sourcing with reliable, ready-to-scale solutions.