Phospho-MKK3-S189+MKK6-S207 Rabbit mAb - P46734 / P52564

Core Product Specifications and Parameters

Biological Background: MKK3/MKK6 Function and Localization

• MKK3 (MAP2K3), also known as MEK3 or SAPKK2, is a dual specificity kinase that phosphorylates both threonine and tyrosine residues on the MAP kinase p38.
• It is activated by pro-inflammatory cytokines and environmental stress, forming part of the stress-activated MAP kinase signaling pathway.
• Upon activation, MKK3 catalyzes the phosphorylation of p38 MAP kinase (MAPK14), which is involved in responses to stress and inflammation.
• MKK3 functions downstream of the adrenergic receptor ADRA1B, linking receptor activation to p38 signaling.
• The kinase is expressed in a wide range of tissues, with particularly abundant expression in skeletal muscle.
• Multiple isoforms exist due to alternative splicing (as referenced by the Swiss-Prot record).
• Post-translational modifications include acetylation and phosphorylation, which regulate its activity.
• Disease-associated variants of MAP2K3 have been identified, linking it to human pathologies.

Experimental Guidance and Technical Tips

• The immunogen was a synthetic phosphorylated peptide around S207 of human MKK3/MKK6; S207 in MKK6 corresponds to S189 in MKK3, the activation site phosphorylated by upstream kinases.
• For phospho-specific detection, use appropriate phosphatase inhibitors during sample preparation.
• Consider validating the antibody in the relevant cellular context under stress stimulation (e.g., cytokines, UV, osmotic shock) to induce phosphorylation.
• Suitable for Western blot (WB), immunohistochemistry (IHC-P), and ELISA; optimize antibody dilutions and detection conditions for each application.
• Since the antibody reacts with human, mouse, and rat, it can be used across these species; confirm endogenous expression levels in target tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development. This product targets phosphorylated stress kinases MKK3/MKK6, which are critical in inflammation and stress signaling research; let us help you secure the raw materials you need for your IVD projects.