Phospho-MEK6-S207 Rabbit pAb - P52564

Core Product Specifications and Parameters

Biological Background: Dual specificity mitogen-activated protein kinase kinase 6 (MEK6) Function and Localization

• Dual specificity protein kinase that is an essential component of the MAP kinase signal transduction pathway, catalyzing phosphorylation of threonine and tyrosine residues in p38 MAP kinases (MAPK11, MAPK12, MAPK13, MAPK14) in response to cytokines and environmental stresses.
• Together with MAP2K3/MKK3, mediates activation of MAPK11 and MAPK13; acts as the major activator of MAPK11 in response to TNF signaling.
• Phosphorylates and activates PAK6, and the downstream p38 MAPK pathway leads to activation of transcription factors such as ATF2 and ELK1, regulating gene expression in stress and inflammatory responses.
• Plays a role in IL-12-induced STAT4 activation via MAPK14, IL-6-induced SOCS3 expression, and IFNG-dependent gene transcription, highlighting its importance in cytokine signaling.
• Contributes to osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and endochondral ossification potentially via SOX9, a downstream target of p38 MAPK.
• Mediates apoptotic cell death in thymocytes and regulates melanocyte dendricity through modulation of Rho family GTPases.
• Subcellular localization includes the nucleus, cytoplasm, and cytoskeleton, indicating diverse signaling platforms.
• Isoform 2 is exclusively expressed in skeletal muscle, while isoform 1 is found in skeletal muscle, heart, and at lower levels in liver and pancreas.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho-peptide corresponding to human MAP2K6 phosphorylated at Serine 207; ensure that the detection condition preserves phosphorylation states (e.g., use phosphatase inhibitors in lysis buffers).
• For Western blot, a band around 37 kDa is expected; consider using a positive control treated with stimuli known to activate the p38 MAPK pathway (e.g., TNF or IL-1β) to confirm phospho-specificity.
• For immunofluorescence, validate fixation and permeabilization conditions as phosphorylation-dependent epitopes may require optimized protocols.
• Perform a phosphatase treatment control (lambda phosphatase) to verify phospho-specific binding in your experimental system.
• Cross-reactivity is reported for human and rat; validate in the relevant species and tissue context.

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