Phospho-MEK1/MEK2-S217/S221 Rabbit mAb for WB, IP, ELISA - Q02750/P36507

Core Product Specifications and Parameters

Biological Background: MEK1/MEK2 Function and Localization

• Dual specificity protein kinases MEK1 (MAP2K1) and MEK2 (MAP2K2) are essential components of the MAPK/ERK signaling cascade, transmitting signals from activated RAS-RAF to ERK1/2.
• Upon activation, MEK1/2 catalyze the concomitant phosphorylation of a threonine and tyrosine residue in the Thr-Glu-Tyr motif of ERK1 (MAPK3) and ERK2 (MAPK1), leading to their activation.
• MEK1/2 are activated by RAF kinases (e.g., RAF1) downstream of growth factor, cytokine, and hormone receptor stimulation, linking extracellular signals to intracellular responses.
• MEK1 also promotes BRAF activation by binding to KSR1 or KSR2 scaffold proteins, releasing an inhibitory interaction and facilitating BRAF dimerization. Related references: PMID:29433126
• The MAPK/ERK pathway regulates diverse biological processes including cell growth, adhesion, survival, and differentiation, as well as transcription, metabolism, and cytoskeletal rearrangements.
• MEK1 localizes to the cytoplasm, cytoskeleton (centrosome, spindle pole), nucleus, and membrane; MEK2 shows similar distribution.
• MEK1 is widely expressed, with extremely low levels in brain. MEK2 has a similar broad expression pattern.
• MEK1/2 are phosphoproteins and serine/threonine/tyrosine kinases; MEK1 phosphorylation at Ser217 and Ser221 is a key activation event.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide targeting phosphorylated Ser217 and Ser221; the antibody is expected to specifically recognize the dually phosphorylated active form of MEK1/2.
• For western blotting, MEK1/2 appear as bands around 40–44 kDa. Use appropriate positive controls (e.g., EGF-stimulated cell lysates) and include phosphatase inhibitors during sample preparation.
• For immunoprecipitation, a rabbit monoclonal antibody offers high specificity and compatibility with standard IP protocols. Validate pull-down efficiency with phospho-ERK1/2 readouts.
• This antibody is suitable for ELISA; consider coating with the phosphopeptide or using a sandwich format for detecting phosphorylated MEK1/2 in cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, contract research organizations, and academic labs with a streamlined one-stop supply chain for IVD raw materials. This phospho-MEK1/2 rabbit monoclonal antibody supports MAPK signaling research and assay development in oncology, neuroscience, and drug discovery. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—covering every stage from concept to clinic.