Phospho-MEK1-T292 Rabbit Polyclonal Antibody for WB and ELISA - Q02750

Core Product Specifications and Parameters

Biological Background: MEK1 Function and Localization

• MEK1 (MAP2K1), also known as PRKMK1, MKK1, is a 43 kDa dual specificity mitogen-activated protein kinase kinase that serves as an essential kinase in the RAS-RAF-MEK-ERK signal transduction cascade.
• Upon activation by RAF1, MEK1 phosphorylates both a threonine and a tyrosine residue within the Thr-Glu-Tyr motif of ERK1 (MAPK3) and ERK2 (MAPK1), leading to their activation and downstream signaling events that regulate cell growth, adhesion, survival, differentiation, transcription, metabolism, and cytoskeletal rearrangements.
• In a KSR1- or KSR2-dependent manner, MEK1 also activates BRAF by releasing inhibitory intramolecular interactions and promoting BRAF dimerization. Related references: PMID:29433126
• MEK1 is involved in the nuclear export of peroxisome proliferator-activated receptor gamma (PPARG) and participates in the regulation of endosomal dynamics, including lysosome processing, endosome cycling through the perinuclear recycling compartment (PNRC), and the fragmentation of the Golgi apparatus during mitosis.
• The protein localizes to multiple subcellular compartments, including the cytoplasm, cytoskeleton (centrosome and spindle pole body), nucleus, and membrane, consistent with its diverse functional roles.
• MEK1 is widely expressed across tissues, with extremely low levels detected in the brain.
• Post-translational modifications such as phosphorylation at threonine 292 (and other sites), acetylation, and alternative splicing regulate its activity and interactions. Mutations in MAP2K1 are associated with diseases like cardiomyopathy, ectodermal dysplasia, and intellectual disability (Noonan syndrome spectrum disorders).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around T292 of human MEK1; this antibody is designed to specifically recognize the phosphorylated form. Treat cells with appropriate stimuli (e.g., growth factors) and include phosphatase inhibitors in lysis buffers to preserve phosphorylation.
• For Western blot, a band around 43 kDa is expected. Use a reducing SDS-PAGE gel and validate detection with positive and negative controls (e.g., phosphatase-treated lysates or non-phosphopeptide blocking).
• The antibody has been shown to work in WB and ELISA applications. It detects both human and rat MEK1. Consider optimizing antibody dilution for your specific samples and detection system.
• When assessing MEK1 phosphorylation dynamics, time-course experiments and pathway inhibitors (e.g., MEK inhibitors) can help confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research laboratories with a comprehensive portfolio of IVD raw materials, including validated phospho-specific antibodies like this anti-Phospho-MEK1-T292 rabbit polyclonal antibody. With expertise in the MAPK signaling pathway, we offer optimized antibody pairs, bulk ancillary reagents, and sourcing of rare targets to accelerate your assay development from concept to clinic.