Phospho-MEK1-T292 Rabbit Monoclonal Antibody for WB, ELISA - Q02750

Core Product Specifications and Parameters

Biological Background: MEK1 Function and Localization

• MEK1 (MAP2K1) is a dual-specificity protein kinase essential for the MAPK/ERK signaling pathway, catalyzing the phosphorylation of threonine and tyrosine residues in ERK1/2 (MAPK3/1) to propagate downstream signals.
• It operates downstream of RAS and RAF1, transducing signals from growth factors, cytokines, and hormones to control cell growth, adhesion, survival, and differentiation.
• MEK1 also promotes BRAF activation by associating with KSR1 or KSR2 scaffold proteins, a mechanism that relieves autoinhibition and enhances dimerization. Related references: PMID:29433126
• The protein localizes to multiple subcellular sites, including the cytoplasm, centrosome, spindle pole, nucleus, and membrane, facilitating its participation in diverse signaling contexts and mitosis.
• MEK1 is broadly expressed with very low abundance in the brain, consistent with its ubiquitous role in regulating fundamental cellular processes.
• Post-translational modifications such as phosphorylation (e.g., at Thr292) and acetylation fine-tune MEK1 activity, and mutations are associated with cardiomyopathy, ectodermal dysplasia, and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to residues surrounding Thr292 of human MEK1 (UniProt Q02750). For Western blot, consider including phosphatase inhibitors in cell lysis buffers to maintain phosphorylation-dependent signals.
• In ELISA, antigen coating with the phosphorylated peptide may require optimization of coating buffer and blocking conditions to ensure phospho-specificity.
• Given the high conservation of MEK1 across human, mouse, and rat, this antibody is expected to detect the phosphorylated form in all three species; however, cross-reactivity should be confirmed experimentally in the relevant species and tissue/cell types.
• For WB, the predicted molecular weight is approximately 43 kDa. Validate the band size using appropriate phospho-site controls (e.g., lambda phosphatase treatment) to confirm specificity.
• As a phospho-specific antibody, lot-to-lot consistency is critical for quantitative applications like ELISA. We recommend titration and parallel testing with non-phospho peptide controls to minimize variability.

CamelBio: Your One-Stop Sourcing Bridge

As a trusted one-stop supplier, CamelBio connects diagnostic manufacturers and laboratories with premium IVD raw materials, technical services, and end-to-end support. For targets like phospho-MEK1 (Thr292) in the MAPK pathway, we offer validated antibody reagents, bulk capacities, and custom sourcing to meet your development and production needs.