Phospho-MEK1-T286 Rabbit pAb - Q02750

Core Product Specifications and Parameters

Biological Background: MEK1 Function and Localization

• Dual specificity protein kinase that is an essential component of the MAP kinase signal transduction pathway, catalyzing the concomitant phosphorylation of a threonine and a tyrosine residue in ERK1/2 (MAPK3/1) on a Thr-Glu-Tyr sequence, leading to their activation.
• Mediates diverse biological functions including cell growth, adhesion, survival and differentiation, predominantly through regulation of transcription, metabolism and cytoskeletal rearrangements.
• In the MAPK/ERK cascade, RAF1 activates MAP2K1/MEK1, which then phosphorylates and activates ERK1/2.
• Activates BRAF in a KSR1 or KSR2-dependent manner, releasing inhibitory intramolecular interactions in KSR proteins and promoting KSR-BRAF dimerization and BRAF activation. Related references: PMID:29433126
• Exports peroxisome proliferator-activated receptor gamma (PPARG) from the nucleus, influencing differentiation and apoptosis.
• Involved in regulation of endosomal dynamics, including lysosome processing and endosome cycling, and in fragmentation of the Golgi apparatus during mitosis.
• Subcellular localization includes cytoplasm, centrosome, spindle pole, nucleus, and membrane.
• Widely expressed in tissues, with extremely low levels in brain.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphorylated peptide around threonine 286 of human MEK1; it is expected to recognize MEK1 only when phosphorylated at T286.
• To preserve phosphorylation, use phosphatase inhibitors in lysis buffers and avoid prolonged sample handling at room temperature.
• Validated applications include Western blot (WB) and ELISA; for WB, a band at approximately 43 kDa is expected. Cross-reactivity has been observed with human, mouse, and rat MEK1.
• Consider validating the antibody in your cell line or tissue of interest under stimulated (e.g., growth factor-treated) versus unstimulated conditions to confirm phospho-specificity.
• For ELISA, titrate the antibody to determine optimal coating and detection conditions; pair with a suitable capture antibody if developing a sandwich assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a streamlined, one-stop supply chain for IVD raw materials. This Phospho-MEK1-T286 Rabbit pAb targets a key node in the MAPK/ERK signaling cascade, relevant to oncology, developmental biology, and cell signaling research. Beyond this product, we support partners with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—bridging your project from concept to clinic.