Phospho-MEK1 (pS221) Rabbit Polyclonal Antibody for WB, ELISA - Q02750

Core Product Specifications and Parameters

Biological Background: MEK1 Function and Localization

• MEK1, encoded by the MAP2K1 gene, is a dual specificity mitogen-activated protein kinase kinase that phosphorylates both threonine and tyrosine residues on its targets ERK1 and ERK2.
• It is a core component of the MAPK/ERK signaling cascade, transmitting signals from activated RAS and RAF to ERK, thereby regulating processes such as cell growth, adhesion, survival, and differentiation.
• MEK1 specifically catalyzes the concomitant phosphorylation of a Thr-Glu-Tyr motif in ERK1/ERK2, leading to their activation and downstream signaling.
• MEK1 also enhances BRAF activation in a KSR1- or KSR2-dependent manner by releasing the autoinhibitory interaction of KSR proteins and promoting KSR-BRAF dimerization. Related references: PMID:29433126
• Subcellularly, MEK1 localizes predominantly to the cytoplasm, centrosome, spindle pole body, and can transiently enter the nucleus; it also associates with membranes.
• MEK1 is widely expressed across tissues, with exceptionally low expression in the brain.
• The protein is subject to acetylation and activating phosphorylation at serine and threonine residues; it is classified as a phosphoprotein and is a reference proteome member.
• Mutations in MAP2K1 are linked to human diseases including cardiomyopathy, ectodermal dysplasia, and intellectual disability.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphorylated peptide corresponding to Ser221 of human MEK1, and is intended to detect MEK1 phosphorylated at this site.
• Validate performance in Western blotting (WB) and ELISA with appropriate phosphorylated and non-phosphorylated controls to confirm phospho-specificity.
• For WB, include phosphatase inhibitors in lysis and running buffers to preserve phosphorylation signals, and consider using total MEK1 antibody as loading control.
• Optimize antibody dilutions experimentally for each application and sample type; preliminary titration is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, from validated antibody pairs and optimized antibodies to bulk ancillary reagents, streamlining your assay development. For researchers and manufacturers focused on MAPK pathway targets, we offer dependable phospho-specific and total antibodies, along with expert sourcing support. Our integrated services cover every step from concept to clinical use, ensuring you have the right tools for your diagnostic or research needs.